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Tissue culture and rapid propagation seedling culture method of paphiopedilum emersonii

A technology for fast-growing seedlings and Paphiopedilum japonicas is applied in horticultural methods, botanical equipment and methods, horticulture, etc., and can solve the problems of slow growth, low rooting rate of seedlings, and low germination rate of P. The rooting rate and germination rate of seedlings are high, and the effect of improving the germination rate of seeds

Inactive Publication Date: 2018-05-04
QIANXINAN LVYUAN ANIMAL SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is: to provide a method for tissue culture and rapid propagation of Papudendron orchid to solve the problems of low seed germination rate, low seedling rooting rate and slow growth, and to speed up the development of Paphiopsis orchid seedlings. growth and reproduction, to meet the needs of large-scale planting of Paphiopedilum

Method used

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Embodiment Construction

[0012] The invention is further described below in conjunction with specific embodiments:

[0013] A method for rapid propagation of seedlings by tissue culture of Paphiopedilum alba, comprising the following steps:

[0014] (1) Disinfection treatment of the seeds of P. albicans: the specific steps are: A. Rinse with soapy water first, and then rinse with sterile water; B. Soak in 0.1% mercuric chloride for 15 minutes, and then rinse with sterile water; C. Finally, soak in 75% alcohol for 10 minutes, and then rinse with sterile water.

[0015] (2) Planting germination culture: the medium used for seed germination culture is 1 / 2MS+20 ml / L coconut milk+20g / L sucrose+20g / L potato+0.1mg / LKT+0.3mg / L activated carbon+6-7g / L agar powder, pH 5.7-5.5, dark culture for 30-60 days, culture temperature 25±1℃.

[0016] (3) Protocorm culture: The medium used for protocorm culture is 1 / 2MS+0.2mg / LBA+20g / L banana+20g / L sucrose+0.3mg / L activated carbon+6-7g / L agar powder, PH5. 7-5.5, light...

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Abstract

The invention discloses a tissue culture and rapid propagation seedling culture method of paphiopedilum emersonii. The method comprises the following steps: paphiopedilum emersonii seed disinfection,seed germination culture, protocorm culture, rooting culture and test-tube seedling transplantation, wherein a medium for seed germination culture contains 1 / 2 MS, 20 ml / L coconut milk, 20 g / L sucrose, 20 g / L potato, 0.1 mg / L KT, 0.3 mg / L activated carbon and 6-7 g / L agar powder, PH is 5.7-5.5, and dark culture is performed for 30-60 days at a temperature of 25 plus or minus 1 DEG C. The medium suitable for tissue culture of the paphiopedilum emersonii is adopted for seed germination culture of the paphiopedilum emersonii, the potato and the active carbon are added to the medium used in the stage, the potato can provide rich nutrients such as starch, protein, crude fiber and the like for seed germination of the paphiopedilum emersonii, seed germination rate and seedling rooting rate of thepaphiopedilum emersonii are increased, besides, the activated carbon can provide a dark environment for seed germination of the paphiopedilum emersonii and adsorbs inhibitors and growth regulators inin-vitro culture, and accordingly, high germination rate and follow-up seedling rooting rate of the paphiopedilum emersonii can be realized.

Description

technical field [0001] The invention relates to a method for tissue-cultured and rapid-propagation seedling cultivation of Papuopsis alba, belonging to the technical field of tissue-cultured seedling cultivation. Background technique [0002] Paphiopedilum is one of the most popular orchid plants with its peculiar flower shape and gorgeous flower color. In the past ten years, due to the destruction of the ecological environment and other reasons, many species have reached the verge of extinction. In order to alleviate the pressure on the collection of wild plants, protect endangered species, and meet people's needs for Paupelopera plants, scholars at home and abroad have conducted a lot of research on the artificial propagation of Paphiopedilum plants. At present, the aseptic sowing and tissue culture techniques of some species of Paphiopedilum have been successful, but the germination rate, seedling rooting rate, slow growth and low survival rate of Paphiopedilum in the ti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 邓克云邓克兰刘森银周丽
Owner QIANXINAN LVYUAN ANIMAL SCI & TECH DEV