Recombinant Coxsackie virus B3 injection and preparation method thereof
A coxsackie virus and injection technology, which is applied in the field of biomedicine, can solve problems such as being susceptible to coxsackie virus B3, and achieve the effect of reducing the probability of neonatal viral myocarditis and preventing viral myocarditis
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Embodiment 1
[0017] Recombinant coxsackie virus B3 injection, which is prepared by the following method:
[0018] S1. Rough treatment of lemons. Take 500g of fresh lemons and place them in a sterile environment, then slice them, remove the liquid produced during the slicing process, and collect fresh lemon slices for later use:
[0019] S2. Concentrate lemons. Take the lemon slices in S1 and add water to cook three times. The first time is 52 minutes, the second time is 42 minutes, and the third time is 32 minutes. Then mix and filter the decoction obtained from the three times of cooking, and remove the decoction. Solid particle impurities in;
[0020] S3, the extract is concentrated for the first time, and the decoction filtered in S2 is distilled under reduced pressure, and the filtrate is concentrated to a density of 1.18g / mL, then adding a concentration of 62% ethanol solution to the filtrate to make the ethanol content 62%, and then carry out Refrigeration treatment, the refrigerati...
Embodiment 2
[0025] Recombinant coxsackie virus B3 injection, which is prepared by the following method:
[0026] S1. Lemon rough treatment, take 600g of fresh lemons and place them in a sterile environment, then slice them, remove the liquid produced during the slicing process, and collect fresh lemon slices for later use:
[0027] S2. Concentrate lemons. Take the lemon slices in S1 and add water to cook three times. The first time is 54 minutes, the second time is 44 minutes, and the third time is 34 minutes. Then mix and filter the decoction obtained from the three times of cooking, and remove the decoction. Solid particle impurities in;
[0028] S3, the extract is concentrated for the first time, and the decoction filtered in S2 is distilled under reduced pressure, and the filtrate is concentrated to a density of 1.20g / mL, then adding a concentration of 64% ethanol solution to the filtrate to make the ethanol content 64%, and then carry out Refrigeration treatment, the refrigeration t...
Embodiment 3
[0033] Recombinant coxsackie virus B3 injection, which is prepared by the following method:
[0034] S1. Rough treatment of lemons. Take 700g of fresh lemons and place them in a sterile environment, then slice them, remove the liquid produced during the slicing process, and collect fresh lemon slices for later use:
[0035] S2. Concentrate lemons. Take the lemon slices in S1 and add water to cook three times. The first time is 56 minutes, the second time is 46 minutes, and the third time is 36 minutes. Then mix and filter the decoction obtained from the three times of cooking, and remove the decoction. Solid particle impurities in;
[0036] S3, the extract is concentrated for the first time, and the decoction filtered in S2 is distilled under reduced pressure, and the filtrate is concentrated to a density of 1.22g / mL, then adding a concentration of 66% ethanol solution to the filtrate to make the ethanol content 66%, and then carry out Refrigeration treatment, the refrigerati...
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