Hand-foot-and-mouth disease pathogen detection primer group and kit

Abstract

The invention discloses a hand-foot-and-mouth disease pathogen detection primer group and a kit, belonging to the technical field of virus detection. The hand-foot-and-mouth disease pathogen detection primer group comprises an RT-LAMP primer of a coxsackie virus type A16 and / or enterovirus type 71. The kit formed by the hand-foot-and-mouth disease pathogen detection primer group comprises a micro-fluidic chip and a detection solution. By adopting the primer system, the amplified reaction background is reduced, and the sensitivity and the specificity are very good. The detection solution system of the kit saves an independent RT-PCR secondary amplification step, so that the detection time is shortened; and moreover, the denaturation and renaturation processes of nucleic acid are avoided, so that the pollution probability of RNA enzyme and amplified nucleic acid is reduced, and the detection sensitivity and the detection safety are improved. By adopting the micro-fluidic chip detection system, the requirement on experimental hardware is lowered, the consumption of a reaction reagent is reduced, the detection cost is decreased, and a detection result can be directly determined by virtue of the color change. The hand-foot-and-mouth disease pathogen detection primer group and the kit have the advantages of safety, specificity, sensitivity and convenience.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a hand, foot and mouth disease pathogen detection primer set and a rapid on-site detection kit for hand, foot and mouth disease based on microfluidic chip technology. Background technique [0002] Hand, foot and mouth disease is a common infectious disease in infants and young children. The onset is characterized by fever, painful oral ulcers, and herpes on the hands, feet and buttocks. HFMD virus can be transmitted through direct contact with nasopharyngeal secretions, saliva, herpes fluid or feces of an infected person. An infected person is most contagious within a week of becoming ill, but the infectious period can last for several weeks. Coxsackievirus A16 (CoxA16 virus) is the most common virus causing HFMD, usually resulting in a mild, self-limited disease, whereas enterovirus 71 (EV71) can cause HFMD, often causing severe complications and even die. [00...

AI Technical Summary

Problems solved by technology

The World Health Organization has formulated and released the "Guidelines for Clinical Management and Public Health Response to Hand, Foot, and Mouth Disease (HFMD)". Due to the wide infectivity of Hand, Foot, and Mouth Disease, the epidemic is difficult to control, and most susceptible people are children under 10 years old. Hand, Foot, and Mouth Disease On-site detection becomes particularly important. There are hand, foot and mouth disease kits based on loop-mediated isothermal amplification technology available on the market, but such products require a strict laboratory environment and are not suitable for on-site sampling and detection.

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