Micro-fluidic chip for single cell catching and cultivation

Abstract

The invention discloses a micro-fluidic chip for single cell catching and cultivation. The micro-fluidic chip comprises an upper sheet and a lower sheet which are combined together, wherein a stop piece array and a microporous array are respectively arranged on the opposite surfaces of the upper sheet and the lower sheet; stop pieces of the stop piece array and micro pores of the microporous arrayare correspondingly distributed in a spacing manner, and are configured that the stop pieces catch single cells in forward liquid flow and prevent the cells in the forward liquid flow from entering the micro pores in front of the stop pieces; backward liquid flow is used for flushing the cells caught by the stop pieces out of the stop pieces and enabling the cells to flow into the micro pores infront of the stop pieces; the stop piece array is located in a micro-fluidic channel formed on the surface of the upper sheet; the micro-fluidic channel is provided with an inlet and an outlet in themicro-fluidic chip. The micro-fluidic chip can simply and efficiently realize catching, arraying and cultivation of the single cells.

Description

technical field [0001] The invention relates to a microfluidic chip for capturing and culturing single cells. Background technique [0002] Single-cell analysis has developed into a key issue in the field of cell biology to study cell function, and limited by tools and technical support, the isolation of single cells and subsequent single-cell studies have become more and more prominent. Traditional methods of extracting single cells are cumbersome, time-consuming, laborious and inefficient. When using a microfluidic chip to manipulate a single cell, it only needs to pass the fluid into the chip, and then perform certain operations to obtain the experimental results. This only takes a few minutes to complete, which can improve the efficiency of the experiment and make the experiment faster and more convenient. By arraying single cells on a microfluidic chip, multiple sets of control experiments can be performed at the same time, which greatly improves the experimental effi...

AI Technical Summary

Problems solved by technology

The capture time of this method is long, and it cannot be effectively combined with cell culture

Researchers in California, U.S.A. use single block arrays to capture single cells, but the success rate of capture is low; and each capture takes 24 hours, so the efficiency is low

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