Plant root freezing slice manufacturing method for synchronous radiation X-ray fluorescence microanalysis
A technology of frozen section and production method, which is applied in the direction of material analysis using wave/particle radiation, preparation of test samples, and analysis of materials. It can solve the problems of easy damage and difficult forming of slices, so as to save time and improve slice efficiency. , solve the fragile effect
Inactive Publication Date: 2018-05-15
INST OF QUALITY STANDARD & DETECTION TECH YUNNAN ACAD OF AGRI SCI
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Problems solved by technology
[0009] In order to solve the technical problem that the existing commercially available OCT embedding agent makes frozen sections of plant roots for step radiation X-ray fluorescence analysis and detection, the slices are not easy to form and easy to break. The invention provides a method for synchrotron radiation X-ray fluorescence Method for making frozen sections of plant roots for microanalysis
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Embodiment 1
[0041] The root of the medicinal plant Panax notoginseng is heavy and solid, the phloem of the fresh Panax notoginseng is flexible, and the central xylem layer is crisp, which is more difficult to make slices than the roots of ordinary plants. The method can be used to prepare fresh frozen sections of the root of Panax notoginseng with complete tissue structure and clear structure. Specific steps are as follows:
[0042] (1) Sample pretreatment:
[0043] Take the main root of 2-year-old fresh Panax notoginseng, wash the sediment, dry the surface moisture of the root of Panax notoginseng with filter paper, cut the rhizome with a root diameter of not less than 10mm, cut it into small pieces with a thickness of 4mm, put the small pieces in Soak in the improved OCT frozen embedding medium at -4°C for 50-120 minutes; the root diameter of Panax notoginseng is 1-5mm, and the soaking time is 50-60min. The time is greater than 60min and less than or equal to 120min (ie, 5mm
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Abstract
The invention discloses a plant root freezing slice manufacturing method for synchronous radiation X-ray fluorescence microanalysis. According to the method, a sample is pretreated, an OTC (optical coherence tomography) freezing embedding agent is improved, the sample is soaked by the improved OTC freezing embedding agent, polyvinyl alcohol, polyethylene glycol and polyvinylpyrrolidone are mixed according to a certain proportion to form the improved OTC freezing embedding agent, the temperature of a slice knife edge is controlled, a user observes whether hardness and softness degrees of the sample and the improved OTC freezing embedding agent are consistent in trimming or not, and the success rate of a fresh plant root slice can reach 100% by prolonging freezing time and suitably improvingtemperature. According to the method, a fresh plant root tissue freezing slice with an integrated structure can be acquired, and a strong technical support is provided for development of synchronousradiation X-ray fluorescence microanalysis of fresh plant root tissues, research of absorption and accumulation micro-zonal distribution of heavy metals, nutrient elements and microelements in plantsand the influence of the heavy metals on physiological ecology of the plants.
Description
technical field [0001] The invention belongs to the technical field of microscopic tissue sectioning, and in particular relates to a method for making frozen sections of plant roots suitable for synchrotron radiation X-ray fluorescence microanalysis. Background technique [0002] Plant roots are the vegetative organs of plants, usually located below the surface, mainly responsible for absorbing water in the soil and dissolving inorganic salts in it, and having the functions of supporting, propagating, storing and synthesizing organic substances. The root system of plants has various forms, generally divided into tap root system, fibrous root system and adventitious root system, etc. The rice root system belongs to the typical fibrous root system. In the process of plant evolution and development, in order to adapt to changes in the environment, the root system has undergone many metamorphosis. There are storage roots with hypertrophy and flesh, such as radish, sweet potato, ...
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IPC IPC(8): G01N23/2202G01N1/36G01N1/42
CPCG01N1/36G01N1/42G01N23/2202
Inventor 陈璐米艳华黎其万杜丽娟
Owner INST OF QUALITY STANDARD & DETECTION TECH YUNNAN ACAD OF AGRI SCI
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