Plant root freezing slice manufacturing method for synchronous radiation X-ray fluorescence microanalysis
A technology of frozen section and production method, which is applied in the direction of material analysis using wave/particle radiation, preparation of test samples, and analysis of materials. It can solve the problems of easy damage and difficult forming of slices, so as to save time and improve slice efficiency. , solve the fragile effect
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[0041] The root of the medicinal plant Panax notoginseng is heavy and solid, the phloem of the fresh Panax notoginseng is flexible, and the central xylem layer is crisp, which is more difficult to make slices than the roots of ordinary plants. The method can be used to prepare fresh frozen sections of the root of Panax notoginseng with complete tissue structure and clear structure. Specific steps are as follows:
[0042] (1) Sample pretreatment:
[0043] Take the main root of 2-year-old fresh Panax notoginseng, wash the sediment, dry the surface moisture of the root of Panax notoginseng with filter paper, cut the rhizome with a root diameter of not less than 10mm, cut it into small pieces with a thickness of 4mm, put the small pieces in Soak in the improved OCT frozen embedding medium at -4°C for 50-120 minutes; the root diameter of Panax notoginseng is 1-5mm, and the soaking time is 50-60min. The time is greater than 60min and less than or equal to 120min (ie, 5mm<the root d...
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