Kit for measuring rT3 content by magnetism particulate chemiluminescence method and detection method of kit

A chemiluminescence method and kit technology, which can be used in measuring devices, biological testing, scientific instruments, etc., and can solve the problems of impossibility and difficulty in coupling methods

Inactive Publication Date: 2018-05-15
TAIZHOU ZECEN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0015] The technical problem to be solved in the present invention is to overcome the luminescent system (enzymatic chemiluminescence) that most of the existing rT3 kits adopt alkaline phosphatase (ALP) or horseradish peroxidase (HRP), but rT3 is directly coupled The method is too difficult or impossible to provide a highly versatile kit that uses rT3 derivatives as raw materials and couples them as anti-reagent components

Method used

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  • Kit for measuring rT3 content by magnetism particulate chemiluminescence method and detection method of kit
  • Kit for measuring rT3 content by magnetism particulate chemiluminescence method and detection method of kit

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Experimental program
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Embodiment

[0056] 1. Preparation process of rT3 antigen derivatives:

[0057] 1) prepare 0.05M MES buffer solution of pH=5.0 and 0.1M NaHCO buffer solution of pH=9.0;

[0058] 2) Weigh 0.5mg of rT3 antigen and dissolve it in 0.05M MES buffer to 5mg / ml;

[0059] 3) Weigh about 3 mg of EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride), and dissolve it to 10 mg / ml with 0.05M MES buffer;

[0060] 4) Prepare 1 ml of ethylenediamine dihydrochloride solution with a final concentration of 5 mg / ml at pH=10.0;

[0061] 5) Add 300ul of the prepared EDC buffer solution to the 5mg / ml rT3 antigen solution, and react at room temperature for 3h;

[0062] 6) Add 0.1ml of ethylenediamine dihydrochloride solution to the system after the reaction in step 5), mix well, and react at room temperature for 1 hour;

[0063] 7) Dialyzing the reaction product in step 6) with a dialysis bag with a molecular weight cut-off of 500, the dialysis buffer is 0.1M NaHCO buffer at pH=9.0;

[0064] 8) Col...

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Abstract

The invention discloses a kit for measuring an rT3 content by a magnetism particulate chemiluminescence method and a detection method of the kit. The kit provides a preparation method of an rT3 antigenic derivative, the rT3 antigenic derivative and an enzyme-labeled rT3 antigenic derivative are prepared, and a biotinylated rT3 antigenic derivative, anti-body enzyme-labeled reagent and calibrationproduct detection system and an enzyme-labeled rT3 antigenic derivative, biotinylated rT3 rabbit polyclonal and calibration product detection system can be built. As an enzymatic luminescence system possibly needs to perform coupling under different conditions such as biotin, enzyme labels and even protein on rT3 antigens under different conditions, the rT3 antigenic derivative serving as an intermediate is prepared, and the problem can be solved. Detection value and given value correlations of different systems are 0.9 or more, good correlations are shown, the correlations of two measuring systems are 0.9540, good correlations of two testing methods are shown, and testing results are consistent, a whole reaction system is short in reaction time, radioactive pollution is avoided, a validity period of a reagent is prolonged, the kit is high in sensitivity and accurate in testing result, and test operation processes are simplified.

Description

technical field [0001] The invention relates to a detection kit, in particular to a kit for measuring rT3 content by a magnetic particle chemiluminescence method and a detection method thereof. Background technique [0002] T3 is an important indicator reflecting thyroid function, which is formed by the removal of an iodine from T4. The structure of rT3 and T3 is basically the same, except that the positions of three iodine atoms are different from T3, at the 3, 3', 5' positions, and in the peripheral tissues ( Such as liver, kidney, etc.) are generated by T4 through the action of 5-deiodinase. rT3 is also an indicator reflecting thyroid function. Serum T4, T3 and rT3 maintain a certain ratio, which can reflect the metabolism of thyroid hormones in the body. [0003] rT3 and T3 are isomers in chemical structure, but T3 is an important hormone involved in the body's metabolism, the process consumes oxygen, while rT3 has almost no physiological activity. The increase of rT3...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/535
CPCG01N33/53G01N33/535G01N2800/046
Inventor 鲁衡刘振世陈海生李婷婷李小艳
Owner TAIZHOU ZECEN BIOTECH CO LTD
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