Biomimetic mineralization material in a polyanion modified fiber, preparation method and application

Abstract

The invention discloses a polyanion modified fiber endobiomimetically mineralized material, a preparation method and application, and particularly relates to a construction method for cross-linking ahigh-molecular-weight polyanion electrolyte on the surface of a collagen scaffold material to form modified collagen, the surface of which carries a functional functional-group, by utilizing a high-molecular-weight polyelectrolyte with an anionic property, such as polyaspartic acid, polyacrylic acid, polyglutamic acid and the like, to simulate the function that non-endocollagen of an organism induces biomineralization and further inducing fiber endobiomimetic mineralization. The method comprises the following steps of treating different types of collagen scaffolds subjected to polyanion surface modification in a calcium phosphate mineralizing solution, so as to obtain a biomimetically mineralized collagen scaffold material with a fiber endomineralization characteristic. According to the preparation method, the fiber endobiomimetic mineralization is induced through the surface modification effect of the high-molecular-weight polyanion electrolyte on the collagen scaffold material, and anovel biomimetically mineralized collagen scaffold material which is similar to a natural bone tissue structure and has excellent mechanical performance and biocompatibility is constructed.

Description

technical field

[0001] The invention belongs to the field of bionic mineralization materials and tissue engineering scaffold materials, and relates to a bionic mineralization material in a polyanion modified fiber, a preparation method and an application. Background technique

[0002] Trauma, infection, tumor, and congenital diseases often cause different types of bone defects. About 2.2 million patients around the world need bone graft repair every year. It is currently the most widely used tissue transplant in clinical practice besides blood transfusion. Autologous bone and allogeneic bone are still the main bone defect repair materials at present. However, their limited sources, immune rejection and other defects have led to the continuous exploration of bone regeneration repair materials. An ideal regenerative repair material should have exactly the same structure and function as the defect tissue. Therefore, one of the current research hotspots in the field of bone rege...

Examples

Embodiment 1

[0044] (1) Dissolve high-molecular-weight anionic electrolyte polyacrylic acid (molecular weight 450kDa) in 2-(N-morpholine)ethanesulfonic acid (MES) buffer to form a polyelectrolyte solution with a concentration of 10mg / mL, and use low-concentration NaOH The solution adjusts the pH of the polyelectrolyte solution to 6.

[0045] (2) 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) was dissolved in the above polyelectrolyte solution at a concentration of 10 mg / mL.

[0046] (3) N-hydroxysuccinimide (NHS) was dissolved in the polyelectrolyte solution at a concentration of 15 mg / mL.

[0047] (4) Standing at room temperature for 15 minutes to activate the carboxyl group on the long-chain polyelectrolyte to form an ester under the action of EDC and NHS.

[0048] (5) Adjust the pH value of the polyelectrolyte solution to 7.0 with a low-concentration NaOH solution.

[0049] (6) Place the collagen sponge or the fully demineralized collagen matrix in the above polyelectrolyte solu...

Embodiment 2

[0062] This embodiment differs from Embodiment 1 in that:

[0063] (1) Dissolve high-molecular-weight polyaspartic acid (180kDa) in 2-(N-morpholine)ethanesulfonic acid (MES) buffer to form a polyelectrolyte solution with a concentration of 5 mg / mL, and use low-concentration NaOH Solution Adjust the pH of the polyelectrolyte solution to 6.0.

[0064] (2) Put the collagen sponge in the polyaspartic acid electrolyte solution, react at room temperature for 2 minutes so that a large number of carboxyl groups can be firmly cross-linked on the collagen molecules through covalent bonding, take out the collagen sponge, and rinse with deionized water repeatedly , 10 minutes each time, repeat 6 times. Then place it on a shaker and vibrate for 20 minutes to completely remove free polyelectrolyte molecules on the surface of the material to obtain a polyanion-modified collagen scaffold material.

Embodiment 3

[0066] This embodiment differs from Embodiment 1 in that:

[0067] (1) Dissolve high-molecular-weight polyglutamic acid (molecular weight 700kDa) in 2-(N-morpholine)ethanesulfonic acid (MES) buffer to form a polyelectrolyte solution with a concentration of 7 mg / mL, and use low-concentration NaOH Solution Adjust the pH of the polyelectrolyte solution to 6.0.

[0068] (2) Put the collagen sponge in the polyaspartic acid electrolyte solution, react at room temperature for 10 minutes so that a large number of carboxyl groups are firmly cross-linked on the collagen molecules through covalent bonding, take out the collagen sponge, and rinse with deionized water repeatedly , 10 minutes each time, repeat 6 times. Then place it on a shaker and vibrate for 20 minutes to completely remove free polyelectrolyte molecules on the surface of the material to obtain a polyanion-modified collagen scaffold material.

technical field

[0001] The invention belongs to the field of bionic mineralization materials and tissue engineering scaffold materials, and relates to a bionic mineralization material in a polyanion modified fiber, a preparation method and an application. Background technique

[0002] Trauma, infection, tumor, and congenital diseases often cause different types of bone defects. About 2.2 million patients around the world need bone graft repair every year. It is currently the most widely used tissue transplant in clinical practice besides blood transfusion. Autologous bone and allogeneic bone are still the main bone defect repair materials at present. However, their limited sources, immune rejection and other defects have led to the continuous exploration of bone regeneration repair materials. An ideal regenerative repair material should have exactly the same structure and function as the defect tissue. Therefore, one of the current research hotspots in the field of bone rege...

Examples

Embodiment 1

[0044] (1) Dissolve high-molecular-weight anionic electrolyte polyacrylic acid (molecular weight 450kDa) in 2-(N-morpholine)ethanesulfonic acid (MES) buffer to form a polyelectrolyte solution with a concentration of 10mg / mL, and use low-concentration NaOH The solution adjusts the pH of the polyelectrolyte solution to 6.

[0045] (2) 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) was dissolved in the above polyelectrolyte solution at a concentration of 10 mg / mL.

[0046] (3) N-hydroxysuccinimide (NHS) was dissolved in the polyelectrolyte solution at a concentration of 15 mg / mL.

[0047] (4) Standing at room temperature for 15 minutes to activate the carboxyl group on the long-chain polyelectrolyte to form an ester under the action of EDC and NHS.

[0048] (5) Adjust the pH value of the polyelectrolyte solution to 7.0 with a low-concentration NaOH solution.

[0049] (6) Place the collagen sponge or the fully demineralized collagen matrix in the above polyelectrolyte solu...

Embodiment 2

[0062] This embodiment differs from Embodiment 1 in that:

[0063] (1) Dissolve high-molecular-weight polyaspartic acid (180kDa) in 2-(N-morpholine)ethanesulfonic acid (MES) buffer to form a polyelectrolyte solution with a concentration of 5 mg / mL, and use low-concentration NaOH Solution Adjust the pH of the polyelectrolyte solution to 6.0.

[0064] (2) Put the collagen sponge in the polyaspartic acid electrolyte solution, react at room temperature for 2 minutes so that a large number of carboxyl groups can be firmly cross-linked on the collagen molecules through covalent bonding, take out the collagen sponge, and rinse with deionized water repeatedly , 10 minutes each time, repeat 6 times. Then place it on a shaker and vibrate for 20 minutes to completely remove free polyelectrolyte molecules on the surface of the material to obtain a polyanion-modified collagen scaffold material.

Embodiment 3

[0066] This embodiment differs from Embodiment 1 in that:

[0067] (1) Dissolve high-molecular-weight polyglutamic acid (molecular weight 700kDa) in 2-(N-morpholine)ethanesulfonic acid (MES) buffer to form a polyelectrolyte solution with a concentration of 7 mg / mL, and use low-concentration NaOH Solution Adjust the pH of the polyelectrolyte solution to 6.0.

[0068] (2) Put the collagen sponge in the polyaspartic acid electrolyte solution, react at room temperature for 10 minutes so that a large number of carboxyl groups are firmly cross-linked on the collagen molecules through covalent bonding, take out the collagen sponge, and rinse with deionized water repeatedly , 10 minutes each time, repeat 6 times. Then place it on a shaker and vibrate for 20 minutes to completely remove free polyelectrolyte molecules on the surface of the material to obtain a polyanion-modified collagen scaffold material.

Images