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Micromolecular polypeptide capable of specifically inhibiting swine fever virus proliferation and application thereof

A technology of small molecule polypeptide and swine fever virus, which is applied in the directions of antiviral agents, peptides, and preparation methods of peptides, can solve the problems of low morbidity and mortality, affect the healthy development of the pig industry, and reduce the lethality, The effect of prolonging the immune protection period and reducing the cytopathic effect

Active Publication Date: 2018-05-29
浙江美保龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, CSF is mostly mild, chronic, and atypical, with low morbidity and mortality, and even occurs in immunized pigs from time to time. Most of them are endemic sporadic epidemics, which seriously affect the healthy development of the pig industry.

Method used

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  • Micromolecular polypeptide capable of specifically inhibiting swine fever virus proliferation and application thereof
  • Micromolecular polypeptide capable of specifically inhibiting swine fever virus proliferation and application thereof
  • Micromolecular polypeptide capable of specifically inhibiting swine fever virus proliferation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: Preparation of a small molecule polypeptide that specifically inhibits the proliferation of classical swine fever virus

[0042] [Met]- [Cys]- [Pro]- [Phe]- [Ile]- [Ala]- [Thr]- [Ala]-[Phe]- [Val]- [Tyr]- [ Arg]-[Arg]-[Cys]-[Ala]-[Thr]-[Lys]-[Cys] (MCPFIATAFVYRRCATKC), the amino acid sequence of which is shown in SEQ ID NO:1. The synthetic small molecular polypeptide is modified by polyethylene glycol modification method, and the terminal hydroxyl group of PEG is converted into a group with higher activity to obtain a PEG active intermediate, and then the PEG active intermediate is used to combine with adjacent hydroxyl groups and α-hydroxyl groups. Acid, β-amino alcohol or α-amino ketone structure reaction of small molecule compounds to obtain polyethylene glycol intermediates; oxidation of polyethylene glycol intermediates with oxidants to polyethylene glycol aldehyde derivatives with active aldehyde groups at the end , and then used for site-directed poly...

Embodiment 2

[0043] Example 2: Preparation of a small molecule polypeptide that specifically inhibits the proliferation of classical swine fever virus

[0044] [Val]- [His]- [Ala]- [Gly]- [Tyr]- [Ser]- [Lys]- [Met]-[Gln]- [Ile]- [Ser]- [ Pro]-[Trp]-[Asp]-[Arg]-[Phe]-[Trp]-[Ala]-[Lys] (VHAGYSKMQISPWDRFWAK), the amino acid sequence of which is shown in SEQ ID NO:2. The synthetic small molecular polypeptide is modified by polyethylene glycol modification method, and the terminal hydroxyl group of PEG is converted into a group with higher activity to obtain a PEG active intermediate, and then the PEG active intermediate is used to combine with adjacent hydroxyl groups and α-hydroxyl groups. Acid, β-amino alcohol or α-amino ketone structure reaction of small molecule compounds to obtain polyethylene glycol intermediates; oxidation of polyethylene glycol intermediates with oxidants to polyethylene glycol aldehyde derivatives with active aldehyde groups at the end , and then used for site-direct...

Embodiment 3

[0045] Example 3: Preparation of a small molecule polypeptide that specifically inhibits the proliferation of classical swine fever virus

[0046][Thr]- [Asp]- [Trp]- [His]- [Cys]- [Glu]- [Trp]- [Tyr]-[Met]- [Ala]- [Ile]- [ Ala]-[Tyr]-[Met]-[Pro]-[Arg]-[Gln]-[Tyr]-[Gln](TDWHCEWYMAIAYMPRQYQ), the amino acid sequence of which is shown in SEQ ID NO:3. The synthetic small molecular polypeptide is modified by polyethylene glycol modification method, and the terminal hydroxyl group of PEG is converted into a group with higher activity to obtain a PEG active intermediate, and then the PEG active intermediate is used to combine with adjacent hydroxyl groups and α-hydroxyl groups. Acid, β-amino alcohol or α-amino ketone structure reaction of small molecule compounds to obtain polyethylene glycol intermediates; oxidation of polyethylene glycol intermediates with oxidants to polyethylene glycol aldehyde derivatives with active aldehyde groups at the end , and then used for site-directed...

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Abstract

The invention relates to micromolecular polypeptide capable of specifically inhibiting swine fever virus proliferation and application thereof and provides structure of the micromolecular polypeptide.The micromolecular polypeptide has the advantages that the micromolecular polypeptide is low in synthesis cost, convenient safe to use and capable of effectively inhibiting proliferation of swine fever virus and can be used with vaccines, thereby greatly improving vaccine antibody valence, prolonging time of antigen in vivo and prolonging immune protection period of vaccines.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a small molecular polypeptide for specifically inhibiting the proliferation of swine fever virus and its application. Background technique [0002] Classical swine fever (classieal swinefever, CSF) is a highly contagious infectious disease caused by swine fever virus, which is widely prevalent in the world. cause major economic losses. The disease often manifests clinical symptoms such as high fever, movement disorders, hind limb weakness, red or purple bleeding spots on the skin, and can also cause a series of complex clinical manifestations, such as abortion in pregnant sows, fetal deformation, stillbirth and weak fetus , Mummified fetus, chronic nutritional wasting disease of piglets. In recent years, CSF is mostly mild, chronic, and atypical, with low morbidity and mortality, and even occurs occasionally in immunized pig herds. [0003] C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C07K1/113A61K38/10A61P31/14
CPCA61K38/00C07K7/08
Inventor 张秀文李阳沈建军
Owner 浙江美保龙生物技术有限公司
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