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Chicken coccidiosis ATG (Autophagy Related Protein) Etatg6 encoding gene sequence and acquiring method thereof

An autophagy-related protein and coding gene technology, which is applied to the coding gene sequence of the autophagy-related protein Etatg6 in chicken coccidia and its acquisition field

Inactive Publication Date: 2018-05-29
INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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Problems solved by technology

All studies have shown that ATG6 plays a vital role in the process of autophagy, but the current research on chicken coccidia is still blank, so in-depth research on chicken coccidia ATG6 is not only for the study of chicken coccidia autophagy The accumulation of data has opened up new prospects for the prevention and control of chicken coccidiosis

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  • Chicken coccidiosis ATG (Autophagy Related Protein) Etatg6 encoding gene sequence and acquiring method thereof

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Embodiment Construction

[0020] In order to make the technical means, creative features, goals and effects of the invention easy to understand, the present invention will be further elaborated below in conjunction with specific illustrations.

[0021] A chicken coccidia autophagy-related protein Etatg6 coding gene sequence and its acquisition method, the material selection day-old Lingnan yellow broiler chicken, 102 full price broiler chicken feed, does not contain any anticoccidial drugs and antibacterial drugs, strains, worm strains and carriers E. tenella (Guangdong strain) oocysts, E. coli DH5α and plasmid vector pMD18-T easy were used respectively, the upstream primer Etatg6-F was 5'ATGGTGGACACAGATTCTCCCCCAGCG3', and the downstream primer Etatg6-R was 5'TTACGGTGCAGAGCCCGCCACCAAG3'.

[0022] The specific method is: step 1, take E.tenella sporulated oocysts, count, dilute to 5.0*10 4 per mL, orally infect 2-4 week-old chickens, inoculate 1 mL each, collect the feces 5 days after inoculation and sep...

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Abstract

The invention discloses a chicken coccidiosis ATG (Autophagy Related Protein) Etatg6 encoding gene sequence and an acquiring method thereof. An encoding gene of a homolog of autophagy related ATG6 isdiscovered through bioinformatics analysis on an E.tenella genome database, clinically isolated E.tenella is used as a model, amplification is successfully realized by utilizing a molecular biologicalmethod, an Etatg6 ORF gene sequence of which the overall length is 1356bp is acquired, and the ATG of which the encoding length is 451 amino acids is discovered through the bioinformatics analysis carried out on the acquired gene sequence, so that data is firstly accumulated for chicken coccidiosis autophagy, and new basis is provided for further screening novel anticoccidial drug targets.

Description

technical field [0001] The invention relates to the technical field of biogenetic engineering, in particular to a gene sequence encoding a chicken coccidia autophagy-related protein Etatg6 and a method for obtaining the same. Background technique [0002] Eimeria tenella (E.tenella) is a kind of obligate intracellular parasitic apicomplexan protozoa, which can cause serious damage to chicken coccidiosis in intensive chicken industry. In the absence of preventive measures or failure of prevention (such as drug ineffective due to drug resistance problems), the incidence rate of chickens can reach 30%-100%, and the lethal rate can be as high as 80%. The global economic loss caused by chicken coccidiosis exceeds more than 3 billion US dollars every year. [0003] At present, the control of chicken coccidiosis is still mainly to implement the technical methods of "adding various anticoccidial drugs to the feed for drug prevention and control" and "live oocyst vaccine for vaccine...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/30C12N15/10
CPCC07K14/455C12N15/1003
Inventor 戚南山林栩慧孙铭飞廖申权吕敏娜吴彩艳李娟胡俊菁刘保臻肖文婉张健騑谢明权
Owner INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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