Microbial agent with high salt resistance, and preparation method and application thereof
A microbial inoculum and salt-tolerant technology, applied in the field of environmental microorganisms, can solve the problems of limited processing capacity and limited application scope, and achieve the effect of broadening the scope of application, improving the processing effect, and strong impact resistance.
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Embodiment 1
[0027] The preparation method of embodiment 1 microbial bacterial agent
[0028] (1) Paracoccus FSTB-2, Microbacterium kitami FSTB-4 and Pseudomonas stutzeri FSTB-5 were inoculated on FSTB solid medium for activation; Staphylococcus FSDN-C, Arthrobacter FDN-1, Flavobacterium waterii FDN-2, Paracoccus denitrificans DN-3 and Methylobacterium SDN-3 were respectively inoculated on the corresponding solid medium for activation; spread evenly and place Activation was carried out in a constant temperature incubator at a temperature of 35°C.
[0029] (2) Use the inoculation loop to inoculate the colonies of Paracoccus FSTB-2, Kitamibacterium FSTB-4 and Pseudomonas stutzeri FSTB-5 on the plate into the corresponding FSTB liquid medium respectively, and use the inoculation loop to Take the colonies of Corkia palustris FSDN-A, Staphylococcus coli FSDN-C, Arthrobacter FDN-1, Flavobacterium waterii FDN-2, Paracoccus denitrificans DN-3 and Methylobacterium SDN-3 on the plate They were res...
Embodiment 2
[0034] Embodiment 2 Microbial agents process saline waste water
[0035] Highly saline wastewater produced by a chemical company has a salt content of 2.5wt%, COD of 2500mg / L, ammonia nitrogen of 20mg / L, total nitrogen of 150mg / L, Ca 2+ Concentration 700mg / L, Mg 2+ The concentration is 500mg / L. During the long-term operation, the removal rate of COD and total nitrogen deteriorated significantly, and the effluent did not meet the discharge requirements. The salt-tolerant microbial bacterial agent prepared in Example 1 was added every day according to 1% of the wastewater treatment volume for a total of 15 days. The treatment effect is shown in Table 2.
[0036] Table 2 Treatment effects of different salt-tolerant bacteria agents
[0037]
[0038] As can be seen from Table 2, after adopting the combined microbial agent prepared by the present invention to treat, the effluent COD concentration is always lower than 60mg / L, the effluent total nitrogen concentration is lower t...
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