Microbial agent with high salt resistance, and preparation method and application thereof

A microbial inoculum and salt-tolerant technology, applied in the field of environmental microorganisms, can solve the problems of limited processing capacity and limited application scope, and achieve the effect of broadening the scope of application, improving the processing effect, and strong impact resistance.

Inactive Publication Date: 2018-06-05
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method mainly relies on denitrification to remove nitrite to deal with COD, the treatment capacity is limited, and the scope of application is also limited to a certain extent.

Method used

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  • Microbial agent with high salt resistance, and preparation method and application thereof
  • Microbial agent with high salt resistance, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation method of embodiment 1 microbial bacterial agent

[0028] (1) Paracoccus FSTB-2, Microbacterium kitami FSTB-4 and Pseudomonas stutzeri FSTB-5 were inoculated on FSTB solid medium for activation; Staphylococcus FSDN-C, Arthrobacter FDN-1, Flavobacterium waterii FDN-2, Paracoccus denitrificans DN-3 and Methylobacterium SDN-3 were respectively inoculated on the corresponding solid medium for activation; spread evenly and place Activation was carried out in a constant temperature incubator at a temperature of 35°C.

[0029] (2) Use the inoculation loop to inoculate the colonies of Paracoccus FSTB-2, Kitamibacterium FSTB-4 and Pseudomonas stutzeri FSTB-5 on the plate into the corresponding FSTB liquid medium respectively, and use the inoculation loop to Take the colonies of Corkia palustris FSDN-A, Staphylococcus coli FSDN-C, Arthrobacter FDN-1, Flavobacterium waterii FDN-2, Paracoccus denitrificans DN-3 and Methylobacterium SDN-3 on the plate They were res...

Embodiment 2

[0034] Embodiment 2 Microbial agents process saline waste water

[0035] Highly saline wastewater produced by a chemical company has a salt content of 2.5wt%, COD of 2500mg / L, ammonia nitrogen of 20mg / L, total nitrogen of 150mg / L, Ca 2+ Concentration 700mg / L, Mg 2+ The concentration is 500mg / L. During the long-term operation, the removal rate of COD and total nitrogen deteriorated significantly, and the effluent did not meet the discharge requirements. The salt-tolerant microbial bacterial agent prepared in Example 1 was added every day according to 1% of the wastewater treatment volume for a total of 15 days. The treatment effect is shown in Table 2.

[0036] Table 2 Treatment effects of different salt-tolerant bacteria agents

[0037]

[0038] As can be seen from Table 2, after adopting the combined microbial agent prepared by the present invention to treat, the effluent COD concentration is always lower than 60mg / L, the effluent total nitrogen concentration is lower t...

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Abstract

The invention relates to a microbial agent with high salt resistance. The microbial agent comprises at least one of selected from a group consisting of Paracoccus sp. FSTB-2, Microbacterium kitamienseFSTB-4 and Pseudomonas stutzeri FSTB-5, also comprises Kocuria palustris FSDN-A and Staphylococcus cohnii FSDN-C, and further comprises at least one of selected from a group consisting of Arthrobacter creatinolyticus FDN-1 and Flavobacterium mizutaii FDN-2. The invention also discloses a preparation method and application of the above microbial agent. The microbial agent can be directly used fortreating total nitrogen and COD in high-salt wastewater, and can also be added to various biochemical reaction structures to improve microbial composition, optimize the salt tolerance of a microbial system in wastewater treatment and enhance the capability of the system in removing total nitrogen and COD.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a high-efficiency salt-tolerant microbial agent and its preparation method and application. The bacterial agent can efficiently remove COD and total nitrogen in saline wastewater, and realize the industrialization and commercialization of the microbial bacterial agent used for the treatment of high-salt wastewater. Background technique [0002] Highly saline wastewater with a salt content higher than 1% from petroleum, chemical, pharmaceutical, and fertilizer industries generally contains high levels of organic pollutants and nitrogen-containing pollutants. The continuous discharge of pollutants in wastewater into the environment will cause serious damage. pollution problem. The shortage of fresh water resources is becoming more and more serious, and the treatment and recycling of waste water have been paid more attention by people. [0003] Physical or c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38C12R1/44C12R1/06C12R1/20C12R1/01
Inventor 高会杰郭志华孙丹凤赵胜楠陈明翔
Owner CHINA PETROLEUM & CHEM CORP
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