Orthopaedic internal fixation fibroin component and preparation method thereof
A technology of silk protein and fixed components, which is applied in the preparation methods of peptides, chemical instruments and methods, medical science, etc., can solve the problems of small batches, long time consumption, large power consumption, etc., and achieves non-toxic immune rejection, superior Mechanical strength and biocompatibility, effect without immune rejection
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[0035] A kind of preparation method of silk protein orthopedic internal fixation component of the present invention comprises:
[0036] Desalination through diffusion dialysis-electrodialysis integrated process to prepare high-purity silk protein solution;
[0037] The silk protein solution is converted into anhydrous silk protein by a vacuum freeze-drying process;
[0038] Anhydrous silk protein is dissolved in hexafluoroisopropanol to obtain a hexafluoroisopropanol solution of silk protein;
[0039] Inject the hexafluoroisopropanol solution of silk protein into the mold, and make silk protein solid through methanol treatment and air drying process;
[0040] Machining of fibroin solids into bone nails and plates.
[0041] Wherein, the preparation method of described silk protein solution is:
[0042] a) Shred silk cocoons and put them in Na 2 CO 3 -NaHCO 3 Boiling for a certain period of time in the buffer solution for degumming to form silk;
[0043] b) The degummed sil...
Embodiment 1
[0055] The invention provides a method for preparing a silk protein orthopedic internal fixation component, and the specific preparation steps are as follows:
[0056] Step 1, prepare silk protein solution.
[0057] a) Shred silk cocoons and put them in Na 2 CO 3 -NaHCO 3 Boil in the buffer solution for a certain period of time, carry out degumming treatment, and form silk; the boiling time is calculated according to the completion time of degumming;
[0058] In this step, under the premise of protecting the inherent structure of silk protein from being damaged, the degumming process parameters can be adjusted to make the degumming more thorough.
[0059] As an example, Na 2 CO 3 -NaHCO 3 The concentration of the buffer solution is 0.1mol / L, the pH value is 10.83, the volume ratio of the cocoon mass to the degumming solution is 100:1g / L, and the boiling time is 30min.
[0060] b) repeatedly washing the degummed silk with deionized water, drying to obtain silk fibers, an...
Embodiment 2
[0080] A method for preparing a silk protein orthopedic internal fixation component, the specific steps are as follows:
[0081] Step 1, prepare silk protein solution.
[0082] a) Shred silk cocoons and put them in Na 2 CO 3 -NaHCO 3 Boiling for a certain period of time in the buffer solution for degumming to form silk;
[0083] As an example, Na 2 CO 3 -NaHCO 3 The concentration of buffer solution is 0.1mol / L, the pH value is 10, the volume ratio of cocoon mass to degumming solution is 100:1g / L, and the boiling time is 30min.
[0084] b) repeatedly washing the degummed silk with deionized water, drying to obtain silk fibers, and loosening for subsequent use;
[0085] As an example, the degummed silk is washed with deionized water, repeated 3 to 5 times, dried in an oven at 60° C., and loosened for later use.
[0086] c) putting the silk fiber into the calcium chloride solution, keeping it warm for a certain period of time, until the solution cannot be seen with the na...
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