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A silk protein orthopedic internal fixation component and its preparation method

A technology of silk protein and fixed components, which is applied in the preparation method of peptides, chemical instruments and methods, medical science, etc., can solve the problems of small batch, long time consumption, large power consumption, etc., and achieve non-toxic immune rejection, superior Effects of mechanical strength and biocompatibility, no immune rejection

Active Publication Date: 2020-08-14
GUANGZHOU ANZE REGENERATIVE MEDICINE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditionally, dialysis bags are used for desalination, which takes a long time, has small batches, and is expensive. There is also a technology that introduces electrodialysis desalination. Although this technology is feasible, it consumes a lot of power due to the high concentration of the salt solution.

Method used

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Examples

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preparation example Construction

[0035] A kind of preparation method of silk protein orthopedic internal fixation component of the present invention comprises:

[0036] Desalination through diffusion dialysis-electrodialysis integrated process to prepare high-purity silk protein solution;

[0037] The silk protein solution is converted into anhydrous silk protein by a vacuum freeze-drying process;

[0038] Anhydrous silk protein is dissolved in hexafluoroisopropanol to obtain a hexafluoroisopropanol solution of silk protein;

[0039] Inject the hexafluoroisopropanol solution of silk protein into the mold, and make silk protein solid through methanol treatment and air drying process;

[0040] Machining of fibroin solids into bone nails and plates.

[0041] Wherein, the preparation method of described silk protein solution is:

[0042] a) Shred silk cocoons and put them in Na 2 CO 3 -NaHCO 3 Boiling for a certain period of time in the buffer solution for degumming to form silk;

[0043] b) The degummed sil...

Embodiment 1

[0055] The invention provides a method for preparing a silk protein orthopedic internal fixation component, and the specific preparation steps are as follows:

[0056] Step 1, prepare silk protein solution.

[0057] a) Shred silk cocoons and put them in Na 2 CO 3 -NaHCO 3 Boil in the buffer solution for a certain period of time, carry out degumming treatment, and form silk; the boiling time is calculated according to the completion time of degumming;

[0058] In this step, under the premise of protecting the inherent structure of silk protein from being damaged, the degumming process parameters can be adjusted to make the degumming more thorough.

[0059] As an example, Na 2 CO 3 -NaHCO 3 The concentration of the buffer solution is 0.1mol / L, the pH value is 10.83, the volume ratio of the cocoon mass to the degumming solution is 100:1g / L, and the boiling time is 30min.

[0060] b) repeatedly washing the degummed silk with deionized water, drying to obtain silk fibers, an...

Embodiment 2

[0080] A method for preparing a silk protein orthopedic internal fixation component, the specific steps are as follows:

[0081] Step 1, prepare silk protein solution.

[0082] a) Shred silk cocoons and put them in Na 2 CO 3 -NaHCO 3 Boiling for a certain period of time in the buffer solution for degumming to form silk;

[0083] As an example, Na 2 CO 3 -NaHCO 3 The concentration of buffer solution is 0.1mol / L, the pH value is 10, the volume ratio of cocoon mass to degumming solution is 100:1g / L, and the boiling time is 30min.

[0084] b) repeatedly washing the degummed silk with deionized water, drying to obtain silk fibers, and loosening for subsequent use;

[0085] As an example, the degummed silk is washed with deionized water, repeated 3 to 5 times, dried in an oven at 60° C., and loosened for later use.

[0086] c) putting the silk fiber into the calcium chloride solution, keeping it warm for a certain period of time, until the solution cannot be seen with the na...

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Abstract

The invention discloses an orthopaedic internal fixation fibroin component and a preparation method of the component. The preparation method comprises the steps of: 1, performing desalination by a diffusion dialysis-electrodialysis integrated technology to prepare a high-purity fibroin solution, 2, converting the fibroin solution into anhydrous fibroin by a vacuum freeze drying technology, 3, dissolving the anhydrous fibroin in hexafluoroisopropanol to form a hexafluoroisopropanol solution of the fibroin, 4, injecting the hexafluoroisopropanol solution of the fibroin into a mold to prepare a fibroin solid by a methanol treatment and airing technology, and 5, machining the fibroin solid into a bone nail and a bone plate. The orthopaedic internal fixation fibroin component prepared by adjusting molecular weight, molecular distribution, crystallization forming parameters and processing parameters has excellent mechanical strength and biocompatibility, is not required to be taken out by secondary operations, is controllable in a degradation rate and free from toxic and immunological rejection effects, and has wide clinical application prospects, and degradation products are amino acidand polypeptide capable of being absorbed by a human body.

Description

technical field [0001] The invention relates to the field of implantable medical devices, in particular to a silk protein orthopedic internal fixation component and a preparation method thereof. Background technique [0002] The orthopedic internal fixation system is one of the most commonly used implantable medical devices in orthopedics. It is used to treat orthopedic diseases that require fixation due to various reasons. Traditional orthopedic internal fixation materials are generally made of stainless steel, titanium and their alloys. Due to their shortcomings such as stress shielding and long-term implantation corrosion releasing metal ions, the clinical demand for new products is very urgent. In recent years, scholars at home and abroad have begun to focus on research on alternative materials for metal internal fixation screws. These internal fixators are gradually absorbed along with bone healing, which not only avoids the stress shielding effect, but also does not r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435A61L31/04A61L31/14C07K1/14
CPCA61L31/047A61L31/14A61L31/148C07K14/43586C08L89/00Y02P20/10
Inventor 王志杰张文张伟黄德球钟杏霞卢梁熠
Owner GUANGZHOU ANZE REGENERATIVE MEDICINE TECH CO LTD
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