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Agarose purification device and method

A purification method and technology of agarose, which is applied in the field of agarose purification equipment, can solve problems such as time-consuming and laborious purification efficiency, and achieve the effects of easy operation and implementation, high purification efficiency, and reduced energy consumption

Inactive Publication Date: 2018-06-08
QINGDAO BRIGHT MOON SEAWEED GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of agarose purification device and method, to solve the problems of time-consuming and laborious and low purification efficiency existing in the method for purifying agarose from agar

Method used

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  • Agarose purification device and method

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Experimental program
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Effect test

Embodiment 1

[0030] (1) Add an agar solution with a mass fraction of 2% into the housing 1 from the inlet 5 and circulate it, and maintain the temperature of the purification device at 80° C. through the heating layer 4;

[0031] (2) Turn on the positive power supply on the positive pole plate 2, and turn on the negative power supply on the negative pole plate 3, so that the sulfate radical in the agar solution is adsorbed on the positive pole plate 2, and the operation duration is 8min;

[0032] (3) Connect the negative power supply on the positive pole plate 2, and connect the positive power supply on the negative pole plate 3, so that the sulfate radicals adsorbed on the positive pole plate 2 come off, and the operation duration is 3min;

[0033] (4) Step (2) and step (3) are repeated, the purified agarose solution is obtained from outlet 6, and the agarose solution is dried to obtain agarose.

[0034] The elemental analyzer was used to monitor the content of sulfate in the agarose solu...

Embodiment 2

[0039] (1) Add the agar solution with a mass fraction of 6% into the housing 1 from the inlet 5 and circulate it, and maintain the temperature of the purification device at 80° C. through the heating layer 4;

[0040] (2) Turn on the positive power supply on the positive pole plate 2, and turn on the negative power supply on the negative pole plate 3, so that the sulfate radical in the agar solution is adsorbed on the positive pole plate 2, and the operation duration is 10min;

[0041] (3) Connect the negative power supply on the positive pole plate 2, and connect the positive power supply on the negative pole plate 3, so that the sulfate radical adsorbed on the positive pole plate 2 falls off, and the operation duration is 3min;

[0042] (4) Step (2) and step (3) are repeated, the purified agarose solution is obtained from outlet 6, and the agarose solution is dried to obtain agarose.

[0043] Make the elemental analyzer monitor the sulfate content in the agarose solution, and ...

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Abstract

The invention discloses an agarose purification device and method. The agarose purification device comprises a casing, a direct current power supply, a plurality of positive electrode plates and a plurality of negative electrode plates, wherein a heating layer used for heating is arranged inside a casing wall of the casing; the positive electrode plate is arranged at one side of the casing; the negative electrode plate is arranged at the other side of the casing; the positive electrode plate and the negative electrode plate are in parallel and crossed arrangement; the direct current power supply provides direct current electricity with variable polarity for the positive electrode plate and the negative electrode plate. The agarose purification device is in a capacitance type; the charge utilization rate is high; the purification efficiency of the agar solution can be improved; the energy consumption is reduced. The agarose purification method has the advantages that the static absorption principle is used; sulfate radicals in the agar solution can be removed; high efficiency and environment-friendly effects are realized; the energy consumption is low; no pollution is caused; the operation and implementation are easy; the purification efficiency is high; the sulfate radical content in the agarose solution obtained after the purification is less than 0.2 percent; the electroendosmosis of the agarose is smaller than 0.13; the purity requirement on the agarose in the fields of molecular biology and the like can be met.

Description

technical field [0001] The invention relates to the technical field of agarose purification, in particular to an agarose purification device and method. Background technique [0002] Agar, also known as agar gel, is a seaweed polysaccharide extracted from Geliflower, Eucheuma, and Gracilaria, and it is composed of agarose and agar ester; among them, agarose does not contain sulfate (salt) as a gelling agent Non-ionic polysaccharide is a gel-forming component, while agar ester is a non-gel part, which is a complex polysaccharide with sulfate (salt), glucuronic acid and pyruvonic acid; general commercial agar contains 2% -7% sulfate ester (salt), 0%-3% pyruvate aldehyde and 1%-3% methyl ethyl. [0003] Agarose is hydrophilic, has almost no charge, and rarely causes denaturation and adsorption to sensitive biomacromolecules. It is an ideal inert carrier. During the preparation of agarose, it is necessary to remove the agar ester as much as possible, otherwise there may be tra...

Claims

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Application Information

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IPC IPC(8): C08B37/12
CPCC08B37/0003C08B37/0039
Inventor 张德蒙张梦雪王鹏王发合秦益民李可昌
Owner QINGDAO BRIGHT MOON SEAWEED GROUP