A kind of preparation method of fermented prawn compound feed
A technology of compound feed and pre-mixed feed, which is applied in animal feed, animal feed, bacteria used in food preparation, etc., can solve problems such as increased resistance of pathogenic microorganisms, decreased diversity of microbial communities, and economic losses in shrimp breeding industry. , to inhibit the growth of harmful bacteria, improve the intestinal environment of shrimp, and improve the effect of adverse effects
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Embodiment 1
[0024] (1) Preparation of liquid fermentation strain
[0025] a. Preparation of Aspergillus niger (purchased from Guangdong Microbial Strain Collection Center, preservation number is GIM 3.576) fermentation broth: place the slant strain of Aspergillus niger in an incubator, activate for 24h at 28°C, pick the slant culture medium Bacterial colony inoculates in the 250mL Erlenmeyer flask that 100mL PDA liquid medium is housed and carries out fermentation culture, culture condition is: temperature 28 ℃, time 24h, obtains Aspergillus niger fermented liquid, the content of Aspergillus niger in the Aspergillus niger fermented liquid is (2.0~4.0 )×10 7 cfu / mL.
[0026] Among them, the formula of PDA liquid medium is: 200g of peeled potatoes, chopped boiled juice, filtered to remove slag, added 20g of sucrose, 10g of peptone, 18g of agar, added water to adjust the volume to 1L, and adjusted the pH to 7.0.
[0027] b. Preparation of Bacillus subtilis (purchased from Guangdong Microbi...
Embodiment 2
[0050] (1) Prepare liquid fermentation strains (Aspergillus niger, Bacillus subtilis, Bacillus licheniformis, Lactobacillus reuteri, Lactobacillus plantarum, Saccharomyces cerevisiae) according to the method of Example 1.
[0051] (2) Take the raw materials according to the following mass fractions: 20% corn dregs, 45% soybean meal, 20% fish meal, 0.8% compound premixed feed for prawns, 14.2% bran, mix the above-mentioned ingredients uniformly to obtain a mixture, and in the mixture Water is added until the water content by weight is 30%, to obtain a solid fermentation medium.
[0052](3) Aerobic fermentation stage: the liquid fermentation strain prepared in step (1) is inoculated into the aspergillus niger fermentation liquid, the Bacillus subtilis fermentation liquid and the Bacillus licheniformis fermentation liquid according to the ratio of 7:2:1 by volume. The solid fermentation medium prepared in step (2), the inoculation amount is mass ratio 10% (the total mass of three...
Embodiment 3
[0058] (1) Prepare liquid fermentation strains (Aspergillus niger, Bacillus subtilis, Bacillus licheniformis, Lactobacillus reuteri, Lactobacillus plantarum, Saccharomyces cerevisiae) according to the method of Example 1.
[0059] (2) take raw material by following mass fraction: corn dregs 30%, soybean meal 30%, fish meal 30%, prawn compound premixed feed 1.5%, bran 8.5%, above-mentioned composition is mixed by its content and obtains mixture, in Water is added to the mixture until its water content by weight is 35%, to obtain a solid fermentation medium.
[0060] (3) Aerobic fermentation stage: the liquid fermentation strain prepared in step (1) is inoculated into the step according to the ratio of Aspergillus niger fermentation liquid, Bacillus subtilis fermentation liquid and Bacillus licheniformis fermentation liquid volume ratio of 8:1:2 (2) The solid fermentation medium prepared, the inoculum size is mass ratio 10% (the total mass of three kinds of bacteria: the quality...
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