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A molecular marker method for identifying non-heading Chinese cabbage Suzhouqing, Bianjiaohuang and Wutaicai

A technology of headed cabbage and molecular marker, which is applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. The effect of short time and simple operation

Active Publication Date: 2021-02-19
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The technical problem to be solved: the present invention is based on the existing non-heading cabbage variety identification and adopts the obvious deficiencies such as large workload, high cost, long time, and result lag in the identification method of planting in field plots. SSR molecular marker technology is used for identification, and It has the advantages of high efficiency, accuracy, not affected by environmental conditions, simple experimental operation, etc.

Method used

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Embodiment 1

[0029] The molecular marker method for identifying non-heading Chinese cabbage Suzhouqing, Bianjiaohuang and Wutaicai provided in this example includes the following steps:

[0030] (1) Extraction of non-heading Chinese cabbage genomic DNA

[0031] DNA was extracted according to the instructions of the Plant Genomic DNA Rapid Extraction Kit (purchased from Hangzhou Baosai Biotechnology Co., Ltd.), briefly as follows:

[0032] 1) Take an appropriate amount of non-heading Chinese cabbage young leaves (100mg) in a 2mL centrifuge tube, add steel balls and place in liquid nitrogen to cool, and use a plant tissue grinder to fully grind;

[0033] 2) Add 500 μL of PL1 preheated at 65°C (beta-mercaptoethanol has been added to 0.2%) and 5 μL of RNaseA, vortex vigorously, and incubate in a water bath at 65°C for 10 minutes;

[0034] 3) Add 170 μL of Buffer PL2 solution, mix thoroughly, place at room temperature for 5 minutes, centrifuge at 12000 rpm for 5 minutes, and transfer the super...

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Abstract

The invention provides a molecular marker method for identifying non-heading Chinese cabbage Suzhouqing, Bianjiaohuang and Wutaicai. The SSR molecular marker primer SSR09133 is designed, and the DNA of non-heading Chinese cabbage is used as a template for amplification, which can The unknown variety of non-heading Chinese cabbage was distinguished from three varieties of Suzhouqing, Aiziaohuang and Wutacai. The method is not affected by the growth and development stage of the plant and the environment, has the advantages of high accuracy, good repeatability, simple operation, and short time-consuming, has high commercial application prospects, and is worthy of wide-scale promotion.

Description

technical field [0001] The invention belongs to the technical field of vegetable variety identification and improved varieties, and in particular relates to a molecular marker method for identifying non-heading Chinese cabbage Suzhouqing, Bianjiaohuang and Wutaicai. Background technique [0002] my country is a big agricultural country and a big seed user. The crop seed industry has become a national strategic and basic core industry, and the quality of seeds is the foundation for promoting the long-term stable development of agriculture and ensuring national food security. Over the past half century, my country has made great achievements in the research of seed industry science and technology. However, with the rapid development of crop breeding, the number of varieties has increased sharply, which poses challenges to the management of the seed market. Field plot planting identification is a legal variety identification method. Although it has the advantages of accuracy a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 刘同坤邵帅旭陈丽张蜀宁张昌伟李英侯喜林
Owner NANJING AGRICULTURAL UNIVERSITY