EGFR and HER2 gene mutation detection kit and detection method, and applications thereof

Abstract

The invention discloses an EGFR and HER2 gene mutation detection kit which includes: an EGFR mutation detection reagent including specific primers, SEQ ID No.1 and SEQ ID No.2, of EGFR gene exons 18,19, 20 and 21 and a dNTP solution; an HER2 mutation detection reagent including specific primers, SEQ ID No.3 and SEQ ID No.4, of HER2 gene exon 20 and a dNTP solution. The invention also discloses amethod of detecting EGFR and HER2 gene mutation by means of the EGFR and HER2 gene mutation detection kit, and applications of the kit. The kit is used for detecting the EGFR and HER2 gene mutation related to lung cancer and other cancers. The kit is quick and accurate to use and easy to operate.

Description

technical field [0001] The invention relates to an EGFR and HER2 gene mutation detection kit, a detection method and an application thereof, belonging to the technical field of gene mutation detection. Background technique [0002] Lung cancer is one of the malignant tumors with the fastest increasing morbidity and mortality and the greatest threat to the health and life of the population. In the past 50 years, many countries have reported that the morbidity and mortality of lung cancer have increased significantly. The incidence and mortality of lung cancer in men rank first among all malignant tumors, and the incidence and mortality of lung cancer occupy the second place in women. According to the degree of differentiation and morphological characteristics of various types of lung cancer, lung cancer is currently divided into two categories, namely small cell lung cancer and non-small cell lung cancer, the latter including squamous cell carcinoma, adenocarcinoma and large ...

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is to overcome the defects of the prior art, provide a kind of EGFR and HER2 gene mutation detection kit, detection method and its application, fast and accurate, easy to operate, adopt 2 pairs of primers to detect the common 29 kinds of EGFR gene Mutations and 3 common mutations of HER2 gene, and other unknown mutations can be detected, which solves the problem of cumbersome and expensive detection procedures of EGFR and HER2 gene mutations in the prior art

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