Construction of evaluation model of rhizoma alismatis blood-lipid regulating quality

A technology for quality evaluation and blood lipid regulation, which is applied to medical preparations containing active ingredients, plant raw materials, instruments, etc., and can solve the problems of not considering the pharmacological effects of blood lipid regulation

Inactive Publication Date: 2018-06-29
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, its quality evaluation is only considered from a chemical point of view, and its active ingredient 23-acetyl alisol B is used as an evaluation index, but its pharmacological and pharmacological effects of regulating blood lipids are not considered.

Method used

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  • Construction of evaluation model of rhizoma alismatis blood-lipid regulating quality
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  • Construction of evaluation model of rhizoma alismatis blood-lipid regulating quality

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Example 1 Preparation of extracts of effective parts of Alisma alisma from different origins

[0011] Weigh 3.00kg of Fujian Alisma decoction pieces, crush them into coarse powder, put them in a round bottom flask, add 5 times the amount of medicinal materials in 95% ethanol, heat and reflux for extraction for 2 hours, filter the extract while it is hot, and repeat the above extraction steps for the remaining residue , Combine the 2 filtrates, recover ethanol, and concentrate in a water bath to form an extract. Dissolve the extract by adding an appropriate amount of water, extract it with petroleum ether and ethyl acetate successively, and recover the reagent from the ethyl acetate part of Alisma alisma, that is, the effective part, to obtain an extract, which is weighed. The extracts of the effective parts of Alisma alisma in Sichuan, Guangdong and Guangxi were prepared by the above method.

[0012] It can be seen from Table 1 that the yields of extracts from the ethy...

Embodiment 2

[0015] Example 2 Blood-lipid-regulating effect of effective parts of Alisma purpura from different origins

[0016] Preparation of fat emulsion: Take 1.00 g of propylthiouracil and grind it finely in a mortar, set aside. Take 20.00g of lard and heat it in a 40°C water bath to melt, put it in a mortar, add 10.00g of cholesterol and 1.00g of propylthiouracil, stir well, and dissolve. Then gradually add 20.00 mL of 10% sodium deoxycholate aqueous solution, and keep stirring, then add 1.00 mL of Tween-80 and 2.00 mL of propylene glycol, grind and emulsify evenly, and finally add distilled water to 100.00 mL. Put it in an airtight container, refrigerate, and thaw in a 37°C water bath before use.

[0017] 90 male ICR mice were randomly divided into 15 groups, 6 in each group. Select one group as the normal control group, feed normally, and feed normal saline 10mL·kg every day. -1 Gavage; the rest of the animals were used for modeling, and 10mL·kg of fat emulsion was gavaged -1 ....

Embodiment 3

[0026] Example 3 Effects of the effective parts of Alisma purpura from four places of origin on the activity of key lipid-regulating enzymes

[0027] In Example 2, fasting for 12 hours after the last administration (without water), after blood was taken from the orbital vein of the mice in each group, the liver was taken from the neck and killed, and an appropriate amount was cut into small pieces, and 1% was added per 1 g of tissue. Sodium lauryl sulfate 9.00mL and phenylmethylsulfonyl fluoride 0.09mL, add reagents respectively, homogenate in ice bath and centrifuge, centrifugation conditions: 4°C, 12000r·min -1 , 15min, aspirate the supernatant and store it at -80°C for later use.

[0028] The supernatant of the liver homogenate was taken, and the activities of the above four enzymes were detected according to the instructions of the HMG-CoA reductase kit, LPL kit, LCAT kit, and ACAT kit.

[0029] SPSS 19.0 statistical software was used to conduct statistical analysis on th...

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Abstract

The invention discloses a construction of an evaluation model of rhizoma alismatis blood-lipid regulating quality. The quality evaluation model with multiple indexes, high efficiency and credibility is structured by carrying out relevance analysis by taking content of rhizoma alismatis blood-lipid regulating effect matter in different producing areas and lipid regulating effect enzymatic activityas an independent variable and the blood lipid parameter index expression level as a dependent variable, and using rhizoma alismatis biological activity as core content and supplementing chemical components as internal indexes. Compared with the qualitative and quantitative analysis of the existing traditional Chinese medicine index components, the model can better objectively and exactly expressthe effect and its quality difference of the traditional Chinese medicine, and provide a new concept and a technical platform for the quality evaluation of the traditional Chinese medicine.

Description

technical field [0001] The invention relates to the construction of a quality model of traditional Chinese medicine, in particular to the construction of a quality model of Alisma alisma. Background technique [0002] Lipid metabolism disorders include abnormal cholesterol and low-density lipoprotein metabolism, abnormal triglyceride-rich lipoprotein metabolism, and abnormal high-density lipoprotein metabolism. Studies have shown that changes in the biological activity of lipoprotein metabolic enzymes can cause changes in lipid metabolism, leading to lipid metabolism disorders and hyperlipidemia. The key enzymes affecting lipid metabolism are trihydroxytrimethylglutaryl coenzyme A (HMG-CoA) reductase, lipoprotein lipase (LPL), lecithin cholesterol acyltransferase (LCAT), lipoacylase A cholesterol ester acyl transferase (ACAT), etc. Under the combined action of LPL, LCAT, LPL, ACAT and other enzymes, it can increase the conversion or metabolism of cholesterol (TC) in the bl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06F19/00A61K36/884A61P3/06
CPCA61K36/884G16C20/50
Inventor 徐飞陆彩吴启南谷巍陈军杜文嘉尤敏
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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