Second-order culture process technique for producing suspended BHK21 cell relevant vaccine

A vaccine production and suspension cell technology, applied in the direction of microorganisms, biochemical equipment and methods, viruses, etc., to achieve the effect of simple production process

Pending Publication Date: 2018-07-03
JIANSHUN BIOSCI CO LTD +1
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Up to now, there is no relevant report on the second-stage culture method of BHK21 suspension cells

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Second-order culture process technique for producing suspended BHK21 cell relevant vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 Second-stage culture process test of BHK21 suspension cells

[0053] 1. Experimental materials

[0054] Cell line: BHK21 cell line derived from ATCC, independently domesticated by Jianshun Biotechnology, can grow in suspension. Among them, the domestication condition is to reduce the serum of BHK21 adherent cells to less than 5%, pass on for more than 3 generations, and then carry out suspension domestication culture. The domestication condition is 5% CO2, and the shaking table speed is 120RPM / min, and the BHK21 cell line capable of suspension growth is screened out. .

[0055] Medium: VM09 serum-free medium (Jianshun Biological JSB (DP126, catalog number: 77013-126).

[0056] MVA-GFP virus (Valneva Company, France).

[0057] 2. Method

[0058] Include the following steps

[0059] (1) Recovery and passage of cells

[0060] (1) Preparation of culture medium: Prepare liquid culture medium VM09 20L according to the instructions, and store it in the dark at ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a second-order culture process technique for producing a suspended BHK21 cell relevant vaccine. According to the second-order culture process technique, a virus capable of pathologically changing on a BHK21 suspended cell is produced by taking the BHK21 suspended cell as a host cell in a second-order culture virus inoculation manner, namely that the BHK21 suspended cell isdiluted by 1.5 times by virtue of a production culture medium when the density grows to a density of 2*10<6> / mL-20*10<6> / mL, the density of the diluted cell is 1*10<6> / mL-10*10<6> / mL, and then virusis inoculated or inoculated before dilution. The production process of the technique is simple; a culture medium is not wasted; the liquid is not replaced; and the production culture medium and a growth culture medium are same or different culture mediums and are mixed in a proportion, so that the purposes of cell growth and virus production are achieved.

Description

technical field [0001] The invention relates to the field of production of vaccines related to suspended BHK21 cells, in particular to a technique for producing vaccines by cultivating suspended BHK21 cells in tanks of 30 L or above and using a second-stage culture method. Background technique [0002] With the transfer of spinner bottle technology and adherent culture to suspension culture and serum-free culture, high-density cell growth and low (suitable) density inoculation have been recognized by the technology in the field of vaccine production. In order to produce qualified vaccines concisely, efficiently and at low cost, the first step is to enable the original adherent cells to grow in suspension. The way to obtain suspension growth is simple domestication, that is, through the change of medium and the change of culture method, the cells that originally adhered to the growth can grow in suspension; similarly, the method of obtaining cell suspension growth is genetic ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00
CPCC12N7/00C12N2710/24134C12N2710/24151
Inventor 罗顺蔡仕君赵志欣王嘉琪
Owner JIANSHUN BIOSCI CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap