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A strain of Bacillus licheniformis, bacterial agent containing the bacterium and application thereof, method and kit for degrading vomitoxin
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A technology of Bacillus licheniformis and deoxynivalenol, which is applied in the field of microorganisms, can solve problems such as the degradation rate needs to be improved and the application range of biological methods is limited, and achieves the effects of high efficiency and rapid degradation, good application prospects, and good stability
Active Publication Date: 2021-06-11
COFCO NUTRITION & HEALTH RES INST +1
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Patent application CN103243047A discloses a strain of Bacillus subtilis that efficiently degrades deoxynivalenol and its application. 900 μL of Bacillus subtilis ANSB471 fermentation broth is reacted with 100 μL of deoxynivalenol (100 μg / ml), and the degradation rate of deoxynivalenol for 2 hours is 25 %, the degradation rate of vomitoxin in 24 hours of reaction is 56%, and the degradation rate of vomitoxin in 48 hours of reaction is 80%, and the degradation rate still needs to be improved
[0005] In addition, most of the existing biological methods for degrading vomitoxin are carried out under mild conditions (such as a temperature of 25-37°C and not exceeding 40°C, and a pH of about 7), however, under higher temperature loads (such as During transport in containers or during feed pelleting) or harsh acidic and alkaline conditions, there is no better solution, which limits the scope of application of biological methods in the degradation of vomitoxin
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[0045] The present invention will be described in detail below by way of examples.
preparation example
[0046] In the following preparation examples, preparation comparative examples, embodiments and comparative examples:
[0047] Deoxynivalenol standard was purchased from Sigma Company, and other experimental materials used were purchased from conventional biochemical reagent stores unless otherwise specified.
[0048] LB liquid medium: 10g peptone, 5g yeast powder, 10g sodium chloride, dilute to 1L with deionized water, adjust pH to 7.
[0049] LB solid medium: 10g peptone, 5g yeast powder, 10g sodium chloride, 16g agar powder, dilute to 1L with deionized water, adjust pH to 7.
[0050] The bacterial strain provided by the present invention is Bacillus licheniformis (Bacillus licheniformis), and was preserved on November 16, 2016 in the General Microbiology Center of China Committee for Microorganism Culture Collection (Address: No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing , Institute of Microbiology, Chinese Academy of Sciences, postal code: 100101) ...
preparation example 1
[0057] After activating the Bacillus licheniformis with the preservation number CGMCC NO.13314 provided by the present invention, inoculate the LB liquid medium sterilized at 121°C for 15 minutes with an inoculum size of 1% by volume, and culture it with shaking at 160 rpm and 37°C , the cultivation time makes the bacterial concentration in the bacterial liquid be (1±0.1)×10 5 CFU / mL. At the same time, it is made into dry powder, and the total number of live bacteria contained in each gram of dry powder is (1±0.1)×10 5 CFU.
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Abstract
The invention relates to the field of microorganisms, and discloses a strain of bacillus licheniformis, a bacterium containing the bacterium and its application, a method for degrading vomitoxin and a kit. Specifically, the present invention provides a strain of Bacillus licheniformis, the preservation number of which is CGMCC NO.13314. The Bacillus licheniformis provided by the present invention can efficiently and rapidly degrade the vomitoxin in the grain, oil and / or feed, especially, even when the content of the vomitoxin in the grain, oil and / or the feed is as high as 50 ppm or more, the Bacillus licheniformis can also efficiently and rapidly The deoxynivalenol can be degraded efficiently without affecting the palatability of grain, oil and / or feed; at the same time, no toxic products are generated by using the Bacillus licheniformis to degrade the vomitoxin, which is safe and environment-friendly. Therefore, the bacillus licheniformis has a good application prospect.
Description
technical field [0001] The invention relates to the field of microorganisms, in particular to a strain of Bacillus licheniformis, a bacterium containing the bacterium and its application, a method and a kit for degrading vomitoxin. Background technique [0002] Vomitoxin (vomitoxin), also known as deoxynivalenol (deoxynivalenol, DON), the chemical name is 3α, 7α, 15-trihydroxy Fusarium-9-en-8-one, because it can cause pigs Named after vomiting, it is mainly trichothecene toxins produced by Fusarium graminearum and Fusarium culmorum infecting wheat, barley, oats, corn and other grains. Worldwide, vomitoxin is one of the main contaminating mycotoxins in grain, feed, and food, seriously affecting the health of humans and livestock. After humans and animals ingest food contaminated by vomitoxin, it will cause acute poisoning symptoms such as anorexia, vomiting, diarrhea, fever, unsteady standing, and unresponsiveness. In severe cases, it will damage the hematopoietic system and...
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