Kit and method for detection of NETs (neutrophil extracellular traps)

A neutrophil and detection method technology, applied in the direction of measuring devices, biological particle analysis, individual particle analysis, etc., can solve the problems of difficult detection and large differences in detection results, and achieve the effect of wide industrial application value

Inactive Publication Date: 2018-08-03
YUANJIAN BIOTECH SHANGHAI CO LTD
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  • Abstract
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  • Application Information

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Problems solved by technology

[0003] However, at present, there is no finished product kit for inducing the formation of neutrophil extracellular traps and quantitatively detecting them in China, which makes it difficult for hospitals, universities, scientific research institutes, and third-party inspection agencies to perform this test. There are a lot of problems, and a lot of pre-experimental conditions are required to explore; and due to different references and other reasons, different institutions may use different experimental methods when testing, so the test results vary greatly

Method used

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  • Kit and method for detection of NETs (neutrophil extracellular traps)
  • Kit and method for detection of NETs (neutrophil extracellular traps)
  • Kit and method for detection of NETs (neutrophil extracellular traps)

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Embodiment 1

[0083] The kit of the present invention can detect the generation ability of NETs under the physiological or pathological conditions of the subject, so as to determine whether the generation of NETs of the subject is abnormal. Usually fresh peripheral venous blood from the subject is required for the test.

[0084] like image 3 As shown, the specific operation steps of this embodiment are as follows:

[0085] First step, isolation of subject's centrioles

[0086] Take 5ml of fresh EDTA peripheral venous blood from the subject and dilute it with PBS at a ratio of 1:1. Take a 50ml centrifuge tube, first add 5ml leukocyte separation solution Histopaque-1119, then carefully add 5ml Histopaque-1077 above the surface of Histopaque-1119, be careful not to mix. Then the diluted subject's blood was slowly trickled along the tube wall and superimposed into the test tube filled with the above separation solution (be careful not to damage the liquid layer), and then centrifuged horizo...

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Abstract

The invention provides a kit and a method for detection of NETs (neutrophil extracellular traps). The NETs are produced by in-vitro simulation induction and subjected to qualitative and quantitative detection. The kit is suitable for detecting NET generating capacity of subjects under physiological or pathological conditions and applied to screening of drugs for promoting or inhibiting generationof NETs and other immunological experiment studies related to the NETs; the kit not only is used for detection of human samples, but also can be used for detection of the NETs of other species such asmice, rats, rabbits, dogs, cattle, pigs and the like, and has wide industrial application value.

Description

technical field [0001] The invention relates to the field of a kit for detecting neutrophil extracellular traps, which can be used to induce the formation of neutrophil extracellular traps and perform quantitative detection thereof, belonging to the field of biomedical research. Background technique [0002] After being stimulated by external pathogenic microorganisms, the normal body resists infection and performs immune defense functions through a series of innate and adaptive immune responses. Among them, innate immunity is the first line of defense acquired by the body innately. It appears early and responds quickly, and plays an important role in the process of eliminating pathogens by the body. Neutrophils are an important part of the innate immune system. In 2004, people discovered a new anti-infection mechanism of neutrophils for the first time, that is, neutrophils release neutrophil extracellular traps (neutrophil extracellular traps, NETs) after being stimulated ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N15/14G01N15/00G01N15/10
CPCG01N33/533G01N15/00G01N15/10G01N15/1434G01N2015/0065G01N2015/1006
Inventor 杜飞
Owner YUANJIAN BIOTECH SHANGHAI CO LTD
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