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Method for promoting differentiation of cultured cells and cultured cell differentiation-promoting agent

A differentiation promoter and a technology for culturing cells, which is applied in the field of culturing cell differentiation promoters to achieve the effect of promoting differentiation

Inactive Publication Date: 2018-08-03
ZEON CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] As mentioned above, the utilization and practical application of stem cells are advancing, but the differentiation and cultivation of stem cells requires a long time of several weeks, and there is a problem of practical application

Method used

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  • Method for promoting differentiation of cultured cells and cultured cell differentiation-promoting agent
  • Method for promoting differentiation of cultured cells and cultured cell differentiation-promoting agent
  • Method for promoting differentiation of cultured cells and cultured cell differentiation-promoting agent

Examples

Experimental program
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Effect test

manufacture example 1

[0067] Using "APEL (registered trademark) APL6013T" manufactured by Mitsui Chemicals Co., Ltd. and "TOPAS (registered trademark) 6013" manufactured by Polyplastics Co., Ltd. as norbornene-based addition polymers, petri dishes with a bottom diameter of 3 cm were molded by injection molding ( The TOPAS dish and the APEL dish were sterilized by the gas method using EOG (ethylene oxide gas) after each being referred to as "TOPAS dish" and "APEL dish" hereafter.

Embodiment 1

[0069] Use DMEM / Ham's F-12 (1:1, v / v), buffer (HEPES; 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), fetal bovine serum, penicillin, streptomycin The culture medium (pH7.4) for adipose-derived stem cells made of prime and amphotericin B was used in TOPAS dishes and APEL dishes at 1×10 4 cells / cm 2 Adipose stem cells (manufactured by ZENBIO) were each inoculated at the cell density of CO 2 Cultured in the incubator for 10 days. Hereinafter, the sample cultured in the TOPAS dish is referred to as the "TOPAS pre-dish culture sample", and the sample cultured in the APEL dish is referred to as the "APEL pre-dish culture sample".

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Abstract

The invention provides a method for promoting the differentiation of cultured cells characterized in that a norbornene addition polymer molded article is brought into contact with cells being cultured, and a cultured cell differentiation-promoting agent comprising a norbornene addition polymer molded article. The invention provides a method promoting differentiation of cultured cells and a cultured cell differentiation-promoting agent used suitably in this method.

Description

technical field [0001] The present invention relates to a method for promoting differentiation of cultured cells and an agent for promoting differentiation of cultured cells. Background technique [0002] In recent years, regenerative medicine using pluripotent cells such as ES cells and iPS cells, stem cells, and mesenchymal stem cells has attracted attention. Pluripotent cells, stem cells are cells in an undifferentiated state that have not undergone differentiation. In regenerative medicine, these undifferentiated cells are differentiated into target organs, tissues, and cells, and patients are treated using the obtained organs, tissues, and cells. For example, adipose stem cells extracted from subcutaneous adipose tissue of a patient can be differentiated into adipocytes via preadipocytes, or differentiated into bone cells or chondrocytes by differentiation-inducing culture. Plastic surgery treatment and cosmetic behaviors using such stem cells have been realized. In ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12N5/074C12N5/0775C12M1/00C12M1/22
CPCC12M1/00C12M1/22C12N5/0667C12N2533/30C12N2500/50C12N5/0653
Inventor 市村直也
Owner ZEON CORP