Efficient Nepal mallotus tissue culture rapid propagation method

Abstract

The invention discloses an efficient Nepal mallotus tissue culture rapid propagation method, and relates to a plant tissue culture technique in molecular biology experiments. The method includes the steps: (1) explant disinfection; (2) new germination induction; (3) bud seedling induction; (4) seedling strengthening; (5) root induction; (6) seedling hardening and transplanting. The method has theadvantages that strains of selectively bred Nepal mallotus with high yield and oil content can be rapidly propagated through tissue culture to obtain a lot of seedlings, entire culture cycle time is short and only needs 2-3 months, a propagation coefficient is high, roots cut by each generation can be repeatedly used for propagation of next generation, roots are dense, healthy and strong, surviving is easily achieved, the survival rate of hardening seedlings is high, a lot of seedlings are excellent and stable in hereditary features and free from virus and accumulated pathogenic bacteria and can be provided, and a foundation is laid for large-scale planting and development of a whole industry chain of Nepal mallotus.

Description

technical field [0001] The invention relates to plant tissue culture techniques in molecular biology experiments, in particular to an efficient rapid propagation method for tissue culture of jacaranda seeds. Background technique [0002] Idesia polycarpa maxium (Idesia polycarpa maxium.) is a perennial woody plant of Idesia polycarpa maxium. It is widely distributed in East Asia. The plant has a beautiful plant shape, strong habit, and rapid growth. It can be widely used in road greening, wood processing, bio-energy and new drug research and development, etc., especially in the fields of grain and oil development and new energy applications. Development and utilization and ecological restoration and protection are of great significance. [0003] The present invention establishes a high-efficiency tissue culture regeneration system of Jatropha on the basis of breeding excellent strains of Jacarina with high yield and high oil content in the early stage, which can provide a l...

AI Technical Summary

Problems solved by technology

[0008] Although the two asexual reproduction techniques of cutting and grafting can better distinguish male and female plants and retain the excellent genetic traits of the mother plant, there are many shortcomings in these two reproduction methods.

First of all, cutting and grafting techniques will introduce viruses or pathogenic bacteria carried by the mother plant, which will easily lead to strain degradation during large-scale production; secondly, both cutting and grafting techniques need to use a large number of branches of the mother plant as cuttings or scions. However, the excellent strains bred cannot provide too many ear sources. Only through the construction of ear picking gardens for many years can a large amount of ear sources required for cutting and grafting be satisfied, which greatly limits the variety approval and promotion of new strains; Third, cutting and grafting techniques have certain requirements on the growth year, length and growth cycle of branches, and are affected by seasons, making it difficult to continue production throughout the year

[0009] In addition, the existing tissue culture technology still has problems such as complicated breeding steps, long breeding cycle, low amplification coefficient and long rooting cycle.

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