Application of arctiin in preparation of antidepressant drug, and antidepressant drug
An antidepressant and arctiin technology, applied in the field of medicine, can solve the problems of high price, significant antidepressant effect, and large side effects of antidepressant drugs, and achieve the effect of reducing costs and prices
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[0038] Example 1 Study on the effect of arctiside and arctigenin on chronic unpredictable depression in mice
[0039] 1. Materials and Reagents
[0040] 1.1 Experimental animals
[0041] 60 SPF grade C57BL / 6 mice, male, body weight (20±2) g, (purchased from Liaoning Changsheng Biotechnology Co., Ltd., license number: SCXK Liao 2015-001).
[0042] 1.2 Experimental drugs
[0043] Arctigoside (Nanjing Chunqiu Bioengineering Co., Ltd., batch number: 20362-31-6, determined by HPLC, purity ≥98%);
[0044] Arctium aglycone (Nanjing Chunqiu Biological Engineering Co., Ltd., batch number: 7770-78-7, determined by HPLC method, purity ≥98%);
[0045] Positive control drug: Sertraline Hydrochloride Tablets (Zhejiang Jingxin Pharmaceutical Co., Ltd., 50mg / tablet, batch number: A1602262);
[0046] 5-HT (serotonin), DA (dopamine), TNF-α (tumor necrosis factor-α), IL-1β (interleukin-1β) kit (Shanghai Enzyme Link Biotechnology Co., Ltd.);
[0047] NO (nitric oxide) kit (Japan Wako Pure Ch...
Example Embodiment
[0096] Example 2 Cytotoxicity test
[0097] Raw264.7 cells in logarithmic growth phase at 2.5.×10 4 The density of cells / well was evenly planted in a sterile 96-well plate, overnight (12h), when the cells grew to 60%, the cells were treated with different concentrations of arctiin and arctigenin, and the cells were treated in an incubator (37°C, 5% CO). 2 ) for 36h, add 10μL of MTT solution (5mg / mL) to each well, incubate for 3.5h, aspirate the medium, add 150μL of dimethyl sulfoxide (DMSO) to each well, and shake for 5min. Absorbance was measured with a microplate reader at a wavelength of 570 nm. Calculate the half-inhibitory concentration (IC) of arctiin and its aglycones 50 ).
[0098] Table 5. IC of arctiin and aglycone on Raw264.7 cells 50 Impact
[0099]
[0100] It can be seen from the data in Table 5 that the 50% inhibitory concentration of arctiside on mice is significantly higher than that of arctigenin, which indicates that arctiside is less toxic than arct...
Example Embodiment
[0101] The preparation of embodiment 3 arctiside sublingual tablet
[0102] Raw materials and their dosage: 5 g of burdock glycosides, 30 g of mannitol, 40 g of lactose, 2 g of sodium carboxymethyl cellulose and 1 g of magnesium stearate.
[0103] Preparation process: drying, crushing and sieving through a 100-mesh sieve for pretreatment, mixing burdockside with mannitol, lactose and sodium carboxymethyl cellulose, adding an appropriate amount of 70% ethanol to make a soft material, and passing through a 14-mesh sieve to make a soft material. Form into granules, blow dry at 55°C for 2 hours, turn once every 0.5h, use 16-mesh and 100-mesh sieves to granulate, add magnesium stearate and mix well, and press the mixture into tablets in a tablet machine, that is, Arctium sublingual tablets were obtained.
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