MiR-223-3p for inhibiting growth and transfer of osteosarcoma

A technology for osteosarcoma and osteosarcoma cells, which can be applied to antineoplastic drugs, medical preparations containing active ingredients, organic active ingredients, etc., and can solve the problems that miR-223-3p has not been studied in detail

Inactive Publication Date: 2018-08-17
GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the specific role of miR-223-3p in osteosarcoma has not yet been specifically studied

Method used

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  • MiR-223-3p for inhibiting growth and transfer of osteosarcoma
  • MiR-223-3p for inhibiting growth and transfer of osteosarcoma
  • MiR-223-3p for inhibiting growth and transfer of osteosarcoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1, miR-223-3p inhibits the growth and metastasis of osteosarcoma

[0059] 1. Preparation of miR-223-3p overexpressing osteosarcoma cell line

[0060] 1. Construction of miR-223-3p overexpression vector PCDH-miR-223-3p

[0061] According to the conventional PCR method, the encoding gene of miR-223-3p shown in the 501-610th positions of SEQ ID No.3 is used as a template, and the upstream primer and the downstream primer are used to carry out PCR amplification, wherein the upstream primer in the PCR primer: 5 '- GGATCC CCTGGCCTCCTGCAGTGCCA-3'; downstream primer: 5'- CTCGAG CTGGTAAGCATGTGCCGCACTT-3'. BamHI and XhoI double-digest the PCR product and the corresponding vector PCDH (product of System Biosciences, product number CD500B1), and insert the target fragment into the vector according to the correct phase. After transformation and screening, positive clones were identified by enzyme digestion, and the expression of various recombinant proteins was analyze...

Embodiment 2

[0080] Example 2, miR-223-3p is closely related to the clinical prognosis of patients with osteosarcoma

[0081] 1. Patients and Specimens

[0082] 133 cases of osteosarcoma and adjacent tissues were evaluated on the basis of the study of pathological and imaging criteria. Clinical information was obtained from patient visit records. Overall survival time was defined as the time from surgery to death. Patient follow-up data were updated monthly. Specimens were divided into two parts: one was immediately frozen in liquid nitrogen and stored at -80°C until RNA extraction, and the other was used for histopathological evaluation. Table 1 lists the clinical and demographic characteristics of the study population. This study was approved by the Institutional Review Board of the Chinese People's Liberation Army General Hospital (Beijing, China) and was performed with the informed consent of the patients.

[0083] Table 1 Correlation between miR-223-3p expression and clinicopatho...

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Abstract

The invention discloses miR-223-3p for inhibiting growth and transfer of osteosarcoma. The invention provides miR-223-3p and any of the following applications of substances for facilitating expressionof miR-223-3p: preparation of products for inhibiting invasion, migration and / or proliferation of osteosarcoma cells; or preparation of products for inhibiting growth and / or transfer of osteosarcoma.Experiments prove that overexpression of miR-223-3p can inhibit proliferation, invasion and migration of the osteosarcoma cells. In addition, it discovers that miR-223-3p is closely related to the overall survival and prognosis of osteosarcoma patients. Therefore, miR-223-3p potentially becomes a prognostic new biomarker and a therapeutic target for the treatment of the osteosarcoma.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to miR-223-3p inhibiting the growth and metastasis of osteosarcoma, in particular to the application of miR-223-3p in the preparation of products for inhibiting the growth and metastasis of osteosarcoma. Background technique [0002] Osteosarcoma is currently the most common malignant bone lesion with a high incidence in children and adolescents worldwide. Currently, the survival rate of osteosarcoma patients with distant metastasis drops to 10%-30%. Recent advances in bioinformatics and other scientific technologies have uncovered some potential treatments for osteosarcoma. However, the clinical outcomes of patients with osteosarcoma have not improved significantly. Therefore, it is crucial to find better clinical treatment options for osteosarcoma and understand the mechanism of action. [0003] MicroRNA (microRNA, miRNA), as a type of endogenous small RNA, can regulate the function ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7105A61K31/7088A61P35/00A61P35/04
CPCA61K31/7088A61K31/7105A61P35/00A61P35/04
Inventor 冀全博王岩梁乙然徐亚梦徐小洁
Owner GENERAL HOSPITAL OF PLA
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