A genotyping detection kit for 23 gene loci of human rh blood type

Abstract

The invention belongs to the technical field of in vitro detection of human nucleic acid, and particularly relates to a genotyping detection kit for human No. 5, No. 19, No. 21 and 23 gene loci of X / Y chromosomes. The present invention first designs a compound PCR amplification system for genotyping 23 gene loci distributed on human chromosomes 5, 19, 21 and X / Y at the same time, wherein the PCR amplification primer set is: SEQ ID No.1 to SEQ ID No.53; the genotyping detection kit of the present invention includes a primer composition container and a PCR reaction mother solution container; the primer composition container contains the primer composition of SEQ ID No.1 to SEQ ID No.53 stock solution. The invention realizes single-tube amplification of 23 gene loci, the gene loci in each fluorescence channel and in different fluorescence channels are amplified evenly, and the typing map is good; and the cost, manpower and time can be significantly saved, and work is improved. efficiency.

Description

technical field [0001] The invention belongs to the technical field of in vitro detection of human nucleic acid, and specifically relates to the detection of the genotype of a polymorphic gene site in human genome DNA, and specifically relates to a method for detecting human No. 5, No. 19, and No. A kit for Rh blood group genotyping detection for 23 gene loci on the No. and X / Y chromosomes. Background technique [0002] According to the presence or absence of Rh factor, Rh negative and Rh positive blood types can be distinguished. This blood group system is called Rh blood group system. Rh is the first two letters of the foreign language name of the rhesus monkey (Rhesus Macacus). In 1940, when scientists such as Landsteiner conducted animal experiments, they found that Rh blood group antigens existed on the red blood cells of rhesus monkeys and most human beings, so it was named. Those who have Rh agglutinogen on red blood cells of human blood are Rh positive. Otherwise ...

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Problems solved by technology

Due to the lack of mature detection methods in China, there is still a lack of systematic research on the molecular background and population distribution of the Rh blood group in the domestic population. However, some studies have pointed out that the genetic mechanism of the Rh blood group in China is diverse and complex. Studies in different regions of China have found that China is Rh(D) negative. Only about 50% of the population is caused by the deletion of the RhD gene, about 20-30% are the diffuse type, of which more than 95% are 1227G>A mutations, and the remaining about 10-20% are weak D phenotypes, of which 60% The above is weak D15 type in weak D, more than 30% is DVI Type3 type in part D

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