Single-cell whole genome amplification system and method based on microfluidic electrospray

Abstract

The invention provides a single-cell whole genome amplification system and method based on microfluidic electrospray. The system includes a microfluidic chip and an alternate-current power supply module, wherein the microfluidic chip is provided with a sample flow channel used for injecting a discrete phase and a continuous phase, the alternate-current power supply module is used for applying alternate-current power to a sample solution in the sample flow channel, and the sample solution in the sample flow channel generates liquid drops under the action of electric field force and liquid force, and the drops are broken into ultra-small droplets under the pulling action of an alternate-current electric field within a certain distance. The microfluidic chip can be used for generating the ultra-small droplets by means of electrospray, and the accuracy of sequencing is improved.

Description

technical field [0001] The invention relates to the field of microfluidic technology, in particular to a single-cell whole-genome amplification system and method based on microfluidic electrospray. Background technique [0002] Water-in-oil emulsified droplet generation technology is an important direction of current life science and industrial production research, and it is also a key link in mainstream biological experiments, institutional testing and industrial production at home and abroad, such as digital PCR, single-cell sequencing, pharmaceutical and Japanese Production with chemicals, etc. Droplet-based microfluidic technology, as an important branch of microfluidic technology, has the advantages of much lower reagent consumption than the volume required for traditional experimental operations, and performs a large number of reactions while keeping the device miniaturized, so it has broader prospects . This microfluidic technology enables independent control of ind...

AI Technical Summary

Problems solved by technology

[0003] In the field of microfluidics at home and abroad, the current traditional droplet generation method relies on channel geometry to control droplet generation, represented by T-junction and flow-focusing, while the commonality of traditional technologies Disadvantages are slow debugging, instability, and low throughput

Emerging technologies use electrohydrodynamic (EHD) methods to achieve electrical control of droplets through electrodes integrated into microdevices. Two representative examples are dielectrophoresis (DEP) and dielectric electrowetting ( EWOD), but at present, such methods have problems such as complex control and limited control range of droplet size.

Images