Simple and efficient visual biosensing signal amplification method

A biosensing and signal amplification technology, applied in the field of nano biosensing, can solve the problems of inability to use on-site analysis and real-time detection, expensive analytical instruments, dependence on prices, etc., to achieve specific portable qualitative and quantitative detection, high analytical sensitivity, The effect of reducing the cost of analysis

Inactive Publication Date: 2018-08-17
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these technologies have problems such as insufficient analytical sensitivity to varying degrees, the need to rely on expensive a

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A low-cost and high-sensitivity immunoassay based on polystyrene microsphere probes embedded with copper nanoparticles for the timed detection of human oncogene protein p190 / bcr-abl (HOP).

[0025] The specific implementation process is as follows:

[0026] (1) Add 60 μL of HOP monoclonal antibody with a concentration of 0.3 mg / mL (prepared in sodium bicarbonate-sodium carbonate buffer solution with a concentration of 10 mmol / L, pH 9.5) to a single enzyme-labeled well on the microplate, 4 Stand overnight in the refrigerator at ℃, absorb and immobilize the antibody on the bottom of the enzyme-labeled well, discard the liquid and dry it, and then fill each well with washing solution (concentration: 10 mmol / L sodium dihydrogen phosphate-dipotassium hydrogen phosphate buffer solution, pH 7), discard unimmobilized mAb molecules after standing for 40 seconds, and repeat this 4 times, invert the wells on a flat roll paper and pat dry.

[0027] (2) Add 60 μL of bovine serum al...

Embodiment 2

[0034] A low-cost and highly sensitive nucleic acid aptamer-based biochemical assay based on polystyrene microsphere probes embedded with copper nanoparticles for the chronometric detection of adenosine.

[0035] The specific implementation process is as follows:

[0036](1) In a 1.5 mL test tube, mix 50 μL of superparamagnetic Fe co-modified with short DNA strands (terminal biotinylated) and adenosine nucleic acid aptamer strands 3 o 4 @SiO 2 Microsphere (~4 μm particle size) suspension solution (prepared with sodium dihydrogen phosphate-dipotassium hydrogen phosphate buffer solution with a concentration of 10 mmol / L, pH 7), 50 μL adenosine sample solution to be tested, 50 μL concentration of 1.5 mg / mL suspension of polystyrene microspheres (~500 nm particle size) labeled with streptavidin and embedded with nano-copper particles (~20 nm particle size) inside (concentration of 10 mmol / L Sodium dihydrogen phosphate-dipotassium hydrogen phosphate buffer solution, pH 7), mixed...

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Abstract

The invention discloses a simple and efficient visual biosensing signal amplification method. The method comprises the following steps: binding a catalytic nano-material physically-embedded functionalized compound probe to the surface of a solid phase platform through a specific molecular recognition reaction by using a target analyte in a sample solution; changing the environmental factors of thesurface of the solid phase platform to release the catalytic nano-material from the functionalized compound probe into the solution; adding a weakly acidic 2-(N-morpholino)ethanesulfonic acid solution and a chloroauric acid solution to the obtained mixed solution to rapidly generate a red nano-gold solution, wherein the formation time of the red nano-gold solution is inversely proportional to theanalyte concentration; and performing signal reading by using a battery-powdered cheap and small timer to realize the low-cost portable quantitative detection of the analyte. The method has the advantages of simplicity in operation, low cost, no professional analytical instruments, and suitableness for onsite analysis and instant detection, and can be directly applied to fields of medical diagnosis, environmental monitoring, food safety and the like.

Description

technical field [0001] The invention belongs to the technical field of nanometer biosensing, and in particular relates to a simple and efficient method for amplifying visualized biosensing signals. Background technique [0002] Biosensing technology is an emerging and active research field developed by the interdisciplinary integration of chemistry, biology, and physics. Since Updike and Hicks developed the first biosensor using enzyme immobilization technology in 1967, scientists have become very interested in biosensing technology. Biosensing technology uses biologically active units (such as proteins, nucleic acids, enzymes, microorganisms, cells, tissues, etc.) Needle, piezoelectric quartz crystal, paper microfluidic analysis device, etc.), an advanced detection technology for analyzing target objects. Its working principle is: after the target substance to be tested is specifically combined with the biorecognition element (bioactive unit), a physical or chemical react...

Claims

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Application Information

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IPC IPC(8): G01N33/543
CPCG01N33/54306
Inventor 聂瑾芳王兵月张云黄锦坤唐贝兮
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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