A kind of method for preparing silk fibroin-xanthan gum hydrogel support

A technology of xanthan gum and silk fibroin, which is applied in the direction of pharmaceutical formulations, medical preparations with non-active ingredients, prostheses, etc., can solve the problems of easy solubility in water, easy damage, poor water resistance and mechanical properties, and achieve low cost Low cost, large amount of embedding, and the effect of improving drug efficacy

Inactive Publication Date: 2019-07-26
WUHAN TEXTILE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the problems of xanthan gum physical hydrogel are: easy to dissolve in water, poor water resistance and mechanical properties, easy to damage
The disadvantage of this method is that the physical interaction between the silk fibroin particles and the hyaluronic acid hydrogel only depends on the cross-linking of chemical bonds, so the safety and mechanical strength of the composite hydrogel need to be further improved.

Method used

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  • A kind of method for preparing silk fibroin-xanthan gum hydrogel support
  • A kind of method for preparing silk fibroin-xanthan gum hydrogel support
  • A kind of method for preparing silk fibroin-xanthan gum hydrogel support

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Disperse 0.125g of silk fibroin powder in 25mL of NaOH aqueous solution with pH=14, stir it magnetically for 2 hours to prepare a silk fibroin suspension with a concentration of 0.5% w / v, add 1.25g of xanthan gum to the above concentration of 0.5% In the w / v silk fibroin suspension, continue magnetic stirring for 12 hours to obtain a uniformly mixed silk fibroin-xanthan gum sol, add 10 mL of 187.5 mg / mL sodium trimetaphosphate to the above silk fibroin-xanthan gum sol , stirred rapidly for 5 minutes to obtain a pre-gel solution, and after cross-linking the pre-gel solution for 4 hours at a temperature of 37 ° C, 10 mL of 12 mg / mL 1-(3-dimethylaminopropyl)-3-ethylcarbodi A mixed solution of imine hydrochloride and 18 mg / mL N-hydroxysuccinimide was added to the above-mentioned silk fibroin-xanthan gum hydrogel cross-linked by sodium trimetaphosphate, and the cross-linking reaction was carried out at 37 °C for 12 hours to obtain Silk fibroin-xanthan gum hydrogel scaffold, ...

Embodiment 2

[0032] Disperse 1.25g of silk fibroin powder in 25mL of NaOH aqueous solution with pH=12, stir it magnetically for 2h and prepare a silk fibroin suspension with a concentration of 5% w / v, add 0.125g of xanthan gum to the above concentration of 5% In the silk fibroin suspension of w / v, continue magnetic stirring for 12h to obtain a uniformly mixed silk fibroin-xanthan gum sol, add 10mL of 75mg / mL sodium trimetaphosphate into the above-mentioned silk fibroin-xanthan gum sol, Stir rapidly for 5 minutes to obtain a pre-gel solution. After cross-linking the pre-gel solution at 37°C for 30 minutes, add 10 mL of 12 mg / mL 1-(3-dimethylaminopropyl)-3-ethylcarbodiethylene A mixed solution of amine hydrochloride and 18 mg / mL N-hydroxysuccinimide was added to the above-mentioned silk fibroin-xanthan gum hydrogel cross-linked by sodium trimetaphosphate, and the cross-linking reaction was carried out at 37 ° C for 48 hours to obtain silk The silk fibroin-xanthan gum hydrogel scaffold, the r...

Embodiment 3

[0034] Disperse 0.75g silk fibroin powder in 25mL NaOH aqueous solution with pH=13, stir magnetically for 2h and prepare a silk fibroin suspension with a concentration of 3% w / v, add 0.75g xanthan gum to the above concentration of 3% In the silk fibroin suspension of w / v, continue magnetic stirring for 12h to obtain a uniformly mixed silk fibroin-xanthan gum sol, add 10mL 225mg / mL sodium trimetaphosphate into the above-mentioned silk fibroin-xanthan gum sol, Stir rapidly for 5 minutes to obtain a pre-gel solution. After cross-linking the pre-gel solution at 37°C for 2 hours, add 10 mL of 12 mg / mL 1-(3-dimethylaminopropyl)-3-ethylcarbodiethylene A mixed solution of amine hydrochloride and 18 mg / mL N-hydroxysuccinimide was added to the above-mentioned silk fibroin-xanthan gum hydrogel cross-linked by sodium trimetaphosphate, and the cross-linking reaction was carried out at 37 ° C for 24 hours to obtain silk The silk fibroin-xanthan gum hydrogel scaffold, the rheological behavio...

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Abstract

The invention discloses a method for preparing a fibroin-xanthan gum aquogel bracket, and belongs to the technical field of natural high polymer materials. The method comprises the following steps: dissolving fibroin powder and xanthan um with different mass ratios into a NaOH aqueous solution, ensuring that an obtained fibroin and xanthan gum solution and sodium trimetaphosphate aqueous solution are subjected to cross-linking reaction for 30 min to 5 h, performing activization through 1-(3-dimethylamino propyl)-3-carbodiimide hydrochloride and N-hydroxy succinimide, and performing continuous and cross-linking reaction for 2 to 48 h at 37 DEG C, so as to obtain the fibroin-xanthan gum aquogel bracket. The method is simple to operate, raw materials adopted are rich in source, a cross-linking agent adopted has water solubility and is nontoxic, a bracket material adopted has controllable capability of drug release, and is good in mechanical property and biocompatibility, and fibroin-xanthan gum aquogel can be used for preparing aquagel fiber, 3D printing artificial tissue brackets and drug-controlled release carriers.

Description

technical field [0001] The invention relates to a method for preparing silk fibroin-xanthan gum hydrogel support. Belonging to the technical field of natural polymer materials, the silk fibroin-xanthan gum hydrogel three-dimensional penetrating porous scaffold can be widely used in industries such as tissue engineering materials, biomedical materials, and drug controllable release. Background technique [0002] Polymer hydrogel has a certain degree of elasticity and a three-dimensional through-porous structure, which is suitable for simulating the extracellular matrix, providing a three-dimensional microenvironment suitable for cell growth and biophysical signals between cells and the extracellular matrix, and maintaining the normal phenotype and physiology of cells. Function. Hydrogel is a three-dimensional network polymer material that can quickly absorb and retain a large amount of water and is insoluble in water. However, hydrogel materials with ordinary structures ofte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/20A61L27/50A61L27/52A61L27/54A61L27/56A61L27/58A61K47/42A61K47/36C08J3/24
CPCA61K9/0002A61K47/36A61K47/42A61L27/20A61L27/227A61L27/50A61L27/52A61L27/54A61L27/56A61L27/58A61L2300/414A61L2300/602A61L2400/06C08J3/246C08J2305/00C08J2389/00C08L5/00
Inventor 陶咏真张如权徐卫林汤舒嵋柏自奎周应山
Owner WUHAN TEXTILE UNIV
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