Recombinant oncolytic adenovirus, recombinant oncolytic adenovirus vector for preparing the recombinant oncolytic adenovirus, construction method and application thereof

An oncolytic adenovirus and vector technology, applied in the field of recombinant oncolytic adenovirus vector and its construction, can solve the problems of inability to fundamentally cure tumors, tumor recurrence and metastasis, inability to remove drug-resistant tumor stem cells, etc., and achieve good application Outlook, efficacy in reducing recurrence and metastasis
CN108424932BActive Publication Date: 2021-01-05BEIJING DOINGTIMES INST OF TRANSLATIONAL MEDICINE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING DOINGTIMES INST OF TRANSLATIONAL MEDICINE
Publication Date
2021-01-05

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Abstract

The invention discloses a recombinant oncolytic adenovirus, a recombinant oncolytic adenovirus vector for preparing the same, a construction method of the recombinant oncolytic adenovirus vector, andapplications of the recombinant oncolytic adenovirus and the recombinant oncolytic adenovirus vector. The construction method of the recombinant oncolytic adenovirus vector comprises following steps:a gene encoding RGD peptide is introduced into adenovirus plasmid, and targeting framework plasmid is formed; a gene sequence of a shRNA anti-tumor stem cell marker molecule ALDH1A1 and a gene sequence of an apoptosis-promoting gene PUMA are connected to first shuttle plasmid, and second shuttle plasmid is formed; a human telomerase reverse transcriptase promoter is connected to the second shuttleplasmid, and third shuttle plasmid is formed; the third shuttle plasmid and the targeting framework plasmid are recombined in escherichia coli to form the recombinant oncolytic adenovirus vector. Therecombinant oncolytic adenovirus vector constructed with the method is linearized and then transfected to virus packaging cells for packaging, amplification and purification, and then the recombinantoncolytic adenovirus is formed, so that tumor stem cells and common tumor cells of multiple malignant tumors can be effectively recognized and killed.
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Description

technical field

[0001] The invention relates to the field of gene therapy, in particular to a recombinant oncolytic adenovirus, a recombinant oncolytic adenovirus vector for preparing the recombinant oncolytic adenovirus, a construction method and application thereof. Background technique

[0002] Malignant tumors are one of the diseases that seriously threaten human life. For most patients with malignant tumors, chemotherapy, radiotherapy, and biological immunotherapy can be used to kill most of the tumor cells, but they cannot fundamentally cure the tumor. Tumors will relapse and metastasize, resulting in the death of patients, because these conventional chemotherapy cannot eliminate drug-resistant cancer stem cells (cancer stem cells, CSCs), and cancer stem cells have high tumorigenicity, self-renewal replication and differentiation capabilities, so Tumors recur and metastasize.

[0003] In recent years, cancer stem cell research and its application in tumor treatment ha...

Claims

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