Human papilloma virus specific T cell receptor and anti-tumor application thereof

Abstract

The invention provides a T cell epitope specific T cell receptor m2P8D TCR of a human papilloma virus E6 gene, a humanized form h2P8D TCR of the T cell epitope specific T cell receptor m2P8D TCR, andantigen binding fragments of the m2P8D TCR and the h2P8D TCR, and provides nucleic acids encoding the m2P8D TCR, vectors containing the vectors, wherein the two T cell receptors are respectively composed of alpha and beta peptide chains, and also provides nucleic acids encoding the two T cell receptors, a vector containing the nucleic acids, a host cell containing the vector, and anti-tumor applications of the two T cell receptors. The invention further provides a T cell epitope specific T cell receptor m2P8D TCR of the human papilloma virus E6 gene, a humanized form h2P8D TCR of the human papilloma virus E6 gene and preparation methods of the T cell epitope specific T cell receptor m2P8D TCR and the humanized form h2P8D TCR. The two specific T cell receptors and the antigen binding fragments thereof can be used as immune effect activators to stimulate the immune response of the body, thereby generating the action effect of resisting tumors and other diseases.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a T cell receptor (TCR) or an antigen-binding fragment thereof capable of specifically recognizing a T cell epitope of a human papillomavirus gene. Background technique [0002] In 2011, cancer surpassed heart disease as the leading cause of death worldwide. The WHO announced in December 2013 that the number of new cancer patients worldwide has exceeded 14 million each year, which is a substantial increase compared with the 2008 statistics of 12.7 million. As of 2020, cancer will cause 9.6 million deaths every year, and about US$1 trillion is invested in cancer diagnosis and treatment worldwide every year. [0003] In the early 1980s, Allison and others determined the gene structure of the αβ T-cell receptor (TCR), which is responsible for recognizing antigens on the surface of T cells. In the late 1980s, Boone, Rosenberg, Old and others found that there are some tumor-speci...

AI Technical Summary

Problems solved by technology

The early gene product E6 protein can combine with p53 protein to form a complex, resulting in the inactivation of p53, thereby hindering the inhibitory effect of p53 protein in the process of cell carcinogenesis

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