Applications of intestinal flora in preparation of drugs for treatment of depression caused by Dcf1 deficiency

A technology of intestinal flora and depression, applied in the application field of intestinal flora in the preparation of drugs for the treatment of depressive behaviors caused by Dcf1 deletion, which can solve the problems of slow onset of psychotherapy and patients not accepting treatment

Inactive Publication Date: 2018-08-24
SHANGHAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But psychotherapy works more slowly, l...

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  • Applications of intestinal flora in preparation of drugs for treatment of depression caused by Dcf1 deficiency
  • Applications of intestinal flora in preparation of drugs for treatment of depression caused by Dcf1 deficiency
  • Applications of intestinal flora in preparation of drugs for treatment of depression caused by Dcf1 deficiency

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Forced swimming

[0023]Modified on the basis of the Porsolt assay, the forced swimming assay was used to examine the depressive behavior of the Dcf1 knockout mouse model and the WT mouse model. Place the mouse model in a transparent cylinder filled with clear water (water depth 35cm, water temperature: room temperature (25±1°C); transparent glass cylinder: diameter 20cm, height 46cm), timed for 6 minutes, and the mouse model was recorded 4 minutes later activity. When the body of the mouse model was observed to move or the limbs moved slightly, the behavior was defined as desperate behavior. Statistical mouse model maintains the time of hopeless behavior ( figure 1 ).

Embodiment 2

[0024] Embodiment 2: Feces collection and detection

[0025] Fecal samples were all obtained from adult male mouse models raised in the SPF animal room. Use sterile tweezers to put the naturally excreted fresh mouse model feces into a sterile dry collection tube, place it on an ice box, and send it back to the laboratory within 30 minutes, and send it to Meiji Biosequencing Company for sequencing. Fecal samples were sequenced using the Illumina MiSeq platform.

Embodiment 3

[0026] Embodiment three: flora analysis

[0027] Species diversity analysis, species composition analysis, comparative analysis of two groups of samples, and species difference analysis were performed on the sequencing results. Through the above analysis and comparison, the purpose is to find out the differences in the microbial composition in the feces of the Dcf1KO mouse model and the WT mouse model and the dominant species in the samples of each group (Fig. 2, 3).

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Abstract

The present invention relates to applications of intestinal flora in preparation of drugs for treatment of depression caused by Dcf1 deficiency. According to the present invention, the manure of the Dcf1 knockout mouse model and the normal mouse model are detected by 16S rRNA gene sequencing, the difference between the intestinal flora compositions of the Dcf1 knockout mouse model and the normal mouse model is compared, and the results show that the intestinal flora compositions of the Dcf1 knockout mouse model and the normal mouse model are different, the normal mouse model transplanted withthe manure of the Dcf1 knockout mouse model shows the depression-like behavior, the depression-like behavior of the Dcf1 knockout mouse model being subjected to bifidobacteria and lactobacillus intragastric administration treatment can be released, and the contents of Glu, GABA and GABA receptors in the brain of the mouse models transplanted with the flora are changed, such that the change in theintestinal flora of the Dcf1 knockout mouse model can induce the depression-like behavior, and can cause the imbalances of neurotransmitters such as Glu and GABA and the dysfunction of GABA receptorsso as to involve the occurrence of depression.

Description

technical field [0001] The present invention relates to the application of intestinal flora in Dcf1-related depressive behavior, especially the application of intestinal flora in the preparation of drugs for treating depressive behavior caused by Dcf1 deletion. Background of the invention [0002] In recent years, depression has become an important mental disease that threatens human health. Its main clinical features are marked and persistent depression and decreased willpower. However, the pathogenesis of depression is very complicated. It is generally believed that many factors such as genetics, neurobiochemistry, neuroendocrine, psychological and social environment are involved in the pathogenesis of depression, but the specific etiology is still unclear. Current research shows that the effective rate of antidepressants is only 60% to 80%, the cure rate is only 30%, and the onset time is 2 to 4 weeks. But psychotherapy works more slowly, leading many patients not to rec...

Claims

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Application Information

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IPC IPC(8): A61K35/747A61K49/00G01N33/53A61P25/24C12Q1/689
CPCA61K35/745A61K35/747A61K49/0008C12Q1/689C12Q2600/158C12Q2600/178G01N33/53A61K2300/00
Inventor 文铁桥王娇李洁周虹梁丹莉张帆刘轩廷钟语嫣
Owner SHANGHAI UNIV
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