Application of atractylenolide I in preparation of anti-hepatocarcinoma drugs
A technique of atractylide, anti-hepatocellular carcinoma, applied in the field of medicine, can solve the problem that there is no anti-hepatocellular carcinoma of Atractylidene I
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[0012] The present invention will be further described below in conjunction with the examples. It should be noted that the following descriptions are only for explaining the present invention and not limiting its content.
[0013] 1. Test material
[0014] BALB / c nude mice, provided by the Animal Experiment Research Center of Zhejiang University of Traditional Chinese Medicine, experimental animal use license: SYXK (Zhejiang) 2013-0184; age: 4-5w; body weight: 18-22g; sex: female; number of animals : 30 pieces in total. Cyclophosphamide: produced by Jiangsu Hengrui Pharmaceutical Co., Ltd., batch number H32020857.
[0015] 2. Test method and content
[0016] 2.1 Animal grouping and modeling
[0017] Tumor model: Human liver cancer cell line HepG2 uses RPMI-164 culture medium containing 10% volume fraction of calf serum, and the tumor cell line is maintained at 37°C and 5% CO 2 Routine culture under experimental conditions, the cells grow in a single layer adherent, when th...
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