Interruptible siphon valve used for centrifugal microfluidic chip and application method thereof
A technology of microfluidic chips and application methods, which is applied in fluid controllers, laboratory containers, chemical instruments and methods, etc., can solve the problems of limited fluid operations and difficult to realize complex fluid operations, and achieve high reliability, Effects that are easy to implement and have wide application prospects
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Embodiment 1
[0043] Embodiment 1. Interruptible siphon valve of centrifugal microfluidic chip
[0044] Such as figure 1 As shown, the interruptible siphon valve for a centrifugal microfluidic chip described in this embodiment includes a sample loading chamber 11, a collection chamber 12, a siphon pipe 13, a vent hole 14 in the sample chamber, a vent hole 15 in the collection chamber, and a siphon tube The channel air hole 16; the sample loading chamber 11 is located closer to the rotation center, and the collection chamber 12 is located farther from the rotation center, and the two are connected by a siphon pipe 13 . The air holes 14, 15, 16 can communicate with the atmosphere, or communicate with each other to form a self-ventilating structure.
[0045] Such as Figures 1 to 3 As shown, in the initial state, the liquid is added into the sample chamber 11 and sealed, the centrifuge is started and rotated at a high speed (such as 2500-6000rpm), and the liquid enters the siphon pipe 13, su...
Embodiment 2
[0046] Embodiment 2. Liquid mixing and releasing structure based on interruptible siphon valve
[0047] Such as Figure 5As shown, the liquid mixing and releasing structure based on the interruptible siphon valve described in this embodiment includes a first sample adding chamber 41, a second sample adding chamber 42, a mixing chamber 43, a collecting chamber 44, and a siphon pipe 45, which can be interrupted. Siphon valve 46. The first sample adding chamber 41 is directly connected to the mixing chamber 43, the siphon pipe 45 is connected to the second sample adding chamber 42 and the mixing chamber 43, and the interruptible siphon valve 46 is connected to the mixing chamber 43 and the collecting chamber 44. The first sample adding chamber 41, the second sample adding chamber 42, the mixing chamber 43, the collecting chamber 44 and the interruptible siphon valve 46 are all provided with vent holes, and the above vent holes can be communicated with the atmosphere or can be co...
Embodiment 3
[0049] Embodiment 3. Repeated cleaning structure based on interruptible siphon valve
[0050] Such as Figure 9 As shown, the repeated cleaning structure based on an interruptible siphon valve in this embodiment includes a sample loading chamber 71 , a cleaning chamber 72 , three collection chambers 73 arranged in parallel, an interruptible siphon valve 74 , and a distribution pipeline 75 . The interruptible siphon valve 74 is connected to the sample loading chamber 71 and the cleaning chamber 72, and the distribution pipeline 75 is connected to the cleaning chamber 72 and three collection chambers 73. The sample loading chamber 71, the cleaning chamber 72, the interruptible siphon valve 74 and the distribution pipeline 75 are all provided with vent holes, and the vent holes can be communicated with the atmosphere, or can communicate with each other to form a self-ventilating structure.
[0051] Such as Figure 9-12 As shown, in the initial state, the liquid is added into th...
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