Polypeptide for distinguishing and detecting PCV2 field-virus antibody and vaccine antibody and application of polypeptide in ELISA detection kit

Abstract

The invention discloses a polypeptide for distinguishing and detecting a PCV2 field virus antibody. The polypeptide comprises one or two polypeptides with polypeptide sequences as shown in SEQ ID NO.1 to SEQ ID NO. 4. The invention also discloses preparation methods for the above-mentioned polypeptide, and a coupled polypeptide and a polypeptide of a polysaccharide carrier, and discloses an ELISAdetection kit containing the polypeptide and a preparation method thereof. The ELISA detection kit for distinguishing and detecting the PCV2 field-virus antibody and the vaccine antibody can judge whether a to-be-detected pig farm has a field circovirus infection risk or not through serologic detection; if a weaned piglet is detected that a PCV2 antibody is positive before immunization with a PCV2 vaccine, and the level of the PCV2 antibody is gradually decreased and the level of the a vaccine antibody is gradually increased after immunization with the PCV2 vaccine, the weaned piglet is indicated have a circovirus infection risk, or a sow is indicated to be infected by field circovirus which is transmitted to the piglet through a maternal antibody.

Description

technical field [0001] The invention relates to the field of immunology, more specifically, to a polypeptide for distinguishing and detecting PCV2 wild virus antibody and vaccine antibody and its application in an ELISA detection kit. Background technique [0002] Porcine circovirus (PCV) is one of the smallest animal viruses known. PCV virus includes two serotypes, PCV-1 and PCV-2. It is generally believed that PCV1 is non-pathogenic and widely exists in pig-derived cells and various tissues in normal pigs; PCV-2 is pathogenic and can cause multiple serotypes in pigs. Systemic dysfunction diseases, such as weaned piglet multisystem failure syndrome (PMWS), porcine respiratory disease syndrome (PRDC), porcine dermatitis and nephrotic syndrome (PDNS), piglet congenital tremor (CT), and pig reproductive system disorders. [0003] According to the sequence analysis results of PCV-2 strains, PCV-2 virus isolates are divided into 5 subtypes, PCV-2a~PCV-2e. Currently, the dominan...

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Problems solved by technology

According to Tong Guangzhi's 2012 research survey, the clinical incidence of circular rings in large-scale pig farms has decreased significantly, but the number of positive rings has increased significantly, indicating that Chinese pig farms are generally infected with circovirus and the immune effect of circular inactivated vaccines is not good

[0005] Antibody detection can be used as an effective means to evaluate the immune effect of vaccines. However, because circovirus is commonly infected in pig farms in my country, and circovirus can also stimulate the immune system of pigs to produce antibodies, it is only necessary to evaluate circovirus by antibody detection. The immune effect of the vaccine is of no practical significance, and the existing PCV-2 antibody detection methods lack the identification means that can distinguish wild virus infection from vaccine immune antibodies. It is of great clinical significance to construct an ELISA detection method that can distinguish circovirus antibodies from vaccine antibodies

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