siRNA capable of inhibiting expression of HSP90 gene in breast cancer cells
A breast cancer cell and gene expression technology, applied in the field of siRNA for inhibiting HSP90 gene expression in breast cancer cells, can solve the problems of lack of siRNA and restricting the application of RNAi
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[0018] Example 1: Construction of siRNA inhibiting HSP90 gene expression in breast cancer cells
[0019] 1. Cell Culture
[0020] The human breast cancer MDA-MB-231 cell line was used for cell culture, which was purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences.
[0021] After the recovery of human breast cancer MDA-MB-231 cell line cells, in DMEM medium (containing 10% fetal bovine serum, 3.7g / L sodium bicarbonate, 1 × 10 5 IU / L penicillin, 100mg / L streptomycin), placed at 37°C, saturated humidity, 5% CO 2 Cultured in an incubator, subcultured after digestion with 0.25% trypsin.
[0022] 2.siRNA design
[0023] For the human Hsp90α gene, use http: / / sidirect2.rnai.jp / design.cgi DESIGNER tool to design 5 pairs of interference sequences, and send them to Beijing Qingke Xinye Biotechnology Co., Ltd. for synthesis.
[0024] The nucleotide sequence of the siRNA targeting HSP90 gene expression and the expression vector of siRNA is as follows:
[0025] ...
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