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Application of trichostatin A in improving the expression of MHC class Ⅱ molecules in macrophages under microgravity

A technology of macrophages and microgravity, which is applied in the measurement/inspection of microorganisms, biochemical equipment and methods, material inspection products, etc., to achieve the effect of improving immunity

Active Publication Date: 2021-08-17
GUILIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no relevant report on the application of Trichostatin A to the expression of MHC class II molecules in macrophages under microgravity

Method used

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  • Application of trichostatin A in improving the expression of MHC class Ⅱ molecules in macrophages under microgravity
  • Application of trichostatin A in improving the expression of MHC class Ⅱ molecules in macrophages under microgravity
  • Application of trichostatin A in improving the expression of MHC class Ⅱ molecules in macrophages under microgravity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] C57 / BL6 mouse peritoneal macrophages were cultured under ground gravity and simulated microgravity for 48 hours respectively, and the expression of cell surface molecules CD80, CD86 and MHC class II molecule I-Ab was detected by flow cytometry.

[0023] For the operation method of the above flow cytometry, please refer to D.L. Spector, R.D. Goldman, L.A. Reinwand. Molecular Cloning Experiment Guide Series - Cell Experiment Guide [M]. Huang Peitang, translated. Beijing: Science Publishing Society, 2003: 118.

[0024] The simulated microgravity adopts the simulated microgravity culture system, which was purchased from Shanghai Equl Company, and the model is RCCS-1. The principle of the system simulating microgravity is the same as the rotary culture system invented by NASA, which has been recognized by international counterparts. The usage method of the simulated microgravity culture system is carried out according to the product manual.

[0025] The culture conditions ...

Embodiment 2

[0028] C57 / BL6 mouse peritoneal macrophages were cultured under ground gravity and simulated microgravity conditions for 48 hours, acetylated histone H3, acetylated histone H4, and internal reference protein glyceraldehyde-3-phosphate dehydrogenase The content was detected by Western blot test.

[0029] For the operation method of the above Western blot test, refer to D.L. Spector, R.D. Goldman, L.A. Reinwand. Molecular Cloning Experiment Guide Series - Cell Experiment Guide [M]. Huang Peitang, translated. Beijing: Science Publishing Society, 2003: 639.

[0030] The simulated microgravity adopts the simulated microgravity culture system, which was purchased from Shanghai Equl Company, and the model is RCCS-1. The principle of the system simulating microgravity is the same as the rotary culture system invented by NASA, which has been recognized by international counterparts. The usage method of the simulated microgravity culture system is carried out according to the product ...

Embodiment 3

[0034] C57 / BL6 mouse peritoneal macrophages were cultured under ground gravity and simulated microgravity conditions for 24 hours. Cells for each condition were divided equally into 2 groups. One group was treated with 50nmol / L Trichostatin A for 24 hours (named as Trichostain A group); the other group was left without any treatment and continued to culture for 24 hours (named as control group). The expression of MHC class Ⅱ molecule I-Ab was detected by reverse transcription-fluorescent quantitative PCR

[0035] For the operation method of the above-mentioned reverse transcription-fluorescent quantitative PCR, refer to C.W. Dieffenbacher, G.S. De Foxler. PCR Technology Experiment Guide [M]. Chong Kang, Qu Lijia, translated. Beijing: Science Press, 2006: 131 ..

[0036] The simulated microgravity adopts the simulated microgravity culture system, which was purchased from Shanghai Equl Company, and the model is RCCS-1. The principle of the system simulating microgravity is th...

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Abstract

The invention discloses an application of Trichostatin A in improving the expression of MHC class II molecules in macrophages under microgravity, and belongs to the technical field of Trichostatin A application. The Trichostatin A can be applied to increase the expression of MHC class II molecules in macrophages under microgravity. The present invention finds that Trichostatin A can increase the expression of MHC class Ⅱ molecules in macrophages under microgravity, thus finding that Trichostatin A has the potential to enhance the immunity of mammals under microgravity.

Description

technical field [0001] The invention relates to an application of Trichostatin A in improving the expression of MHC class II molecules of macrophages under microgravity, and belongs to the technical field of Trichostatin A application. Background technique [0002] Trichostatin A (TSA) is a natural product isolated from a species of Streptomyceshygroscopicus by Japanese scientist Tsuji in 1976. Tsuji found that the natural product has an antifungal effect. In 1985, Yoshida and Morioka found that Trichostatin A can induce differentiation of various tumor cells such as human leukemia and human head and neck tumors, and cause tumor cell apoptosis. In 1990, Yoshida et al. discovered that Trichostatin A can affect transcription to interfere with the cell cycle and inhibit the proliferation of tumor cells. [0003] Major histocompatibility complex (MHC) class II molecules are cell membrane surface molecules of antigen-presenting cells such as macrophages, dendritic cells, and B l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888G01N33/53G01N33/68
CPCC12Q1/6888C12Q2600/158G01N33/53G01N33/68
Inventor 王重振张昌良邢婉琳何玉林黄大林袁树民韦晗宁陈建宏周亚莉金科乔冠华蒋莲秀黄光玲吴丹何义
Owner GUILIN MEDICAL UNIVERSITY
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