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Detection method for content of eggs in head-broken noodles and application thereof

A detection method and a worm egg technology are applied in the field of detection of worm egg content in broken heads and noodles, which can solve the problems of detection of worm eggs, the inability to continue recycling and utilization of broken heads and noodles, waste of resources, etc., and achieve the effect of easy operation.

Pending Publication Date: 2018-09-04
想念食品股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] One of the purposes of the present invention is to provide a detection method for the content of worm eggs in crushed noodles, so as to alleviate the fact that there is currently no method for detecting worm eggs in crushed noodles, which leads to the inability to continue to recycle most of the crushed noodles, resulting in a waste of resources technical issues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0078] In a preferred embodiment of the present invention, the preparation method of the standard curve of egg concentration and absorbance comprises the following steps:

[0079] (A) Provide worm egg enrichment solutions with different worm egg concentrations, and grind and break them separately;

[0080] (B) adding chitinase to the egg enrichment liquids with different egg concentrations after grinding and crushing to obtain enzymatic hydrolyzates with different egg concentrations;

[0081] (C) Add dimethylaminobenzaldehyde to the enzymatic hydrolyzate of eggs with different egg concentrations to obtain different color developing solutions, and measure the absorbance of different color developing solutions respectively, with the egg concentration as the abscissa and the absorbance as the Draw the standard curve of egg concentration and absorbance as the ordinate.

[0082] It should be noted that the specific operations of steps (B) and (C) in the preparation of the above st...

Embodiment 1

[0091] The present embodiment provides a kind of detection method of worm egg content in the crushed noodles, comprising the following steps:

[0092] (A) Draw the standard curve of egg concentration and absorbance

[0093] (A1) Preparation of egg enrichment solutions with different egg concentrations: Take 20 adults and put them into 1 kg of flour. After one month, sieve to remove the adults, collect the flour, and add the flour to distilled water at 4°C according to the liquid-to-material ratio of 10:1. In the medium, soak for 4 hours at 4°C, then stir at a speed of 100r / min until the flour fully absorbs water, swells, and disperses in water; then stop stirring, let it stand for 30-40min, take the supernatant, and use it at 800r / min Centrifuge for 8 minutes to precipitate the remaining flour particles in the supernatant, and take the supernatant to obtain the egg enrichment solution; take 1 mL of the above egg enrichment solution and count it under a microscope by traditiona...

Embodiment 2

[0108] This embodiment provides a method for detecting the content of worm eggs in crushed noodles. The difference between this embodiment and Example 1 is that the liquid-to-solid ratio of crushed noodles to distilled water in step (B) is 20:1.

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Abstract

The invention provides a detection method for the content of eggs in head-broken noodles and application thereof, relating to the technical field of detection methods. The detection method comprises the following steps: (a) soaking the head-broken noodles into distilled water, and carrying out solid-liquid separation, so as to obtain egg enriched liquid; (b) carrying out collection and purification on the egg enriched liquid, so as to obtain egg purified liquid; (c) adding chitinase into the egg purified liquid, so as to obtain egg enzymolysis liquid; and (d) adding dimethylaminobenzaldehyde into the egg enzymolysis liquid to generate color-developing reaction so as to obtain a color-developing solution, and determining the absorbancy of the color-developing solution, so as to obtain the content of eggs in head-broken noodles. According to the detection method, the technical problem that the resources are wasted because no special method for detecting the eggs in the head-broken noodles is not reported at present is solved; and the content of the eggs in the head-broken noodles can be accurately determined by virtue of the detection method provided by the invention, and the detection method provides the basis for the recycling of the head-broken noodles.

Description

technical field [0001] The invention relates to the technical field of detection methods, in particular to a detection method and application of insect egg content in crushed noodles. Background technique [0002] Dried noodles are dry noodles with a certain length, which are made by adding salt and water to wheat flour, hanging and drying, and then cutting. The main varieties of dried noodles are ordinary dried noodles, colored dried noodles, handmade dried noodles, etc. Edible dried noodles have the effects of improving anemia, enhancing immunity, and balancing nutrient absorption. [0003] When processing dried noodles, it is necessary to carry out processes such as pretreatment of raw and auxiliary materials, kneading, aging, sheeting, cutting, wet cutting, drying, cutting, measuring, packaging, etc. In the production process of dried noodles, dried noodles need to enter the cutting process. In the process of cutting dried noodles, some broken ends are usually produced....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/78
CPCG01N21/31G01N21/78
Inventor 孙君庚郭媛媛董金龙阴志刚
Owner 想念食品股份有限公司
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