InDel labeling primer pair used for distinguishing five capsicum cultispecies and application thereof

A technology for labeling primers and cultivars, applied in the field of agricultural biotechnology engineering, can solve the problems of low repeatability, difficult classification, easy to be affected by the environment, etc., and achieve the effects of saving manpower, simple belt shape, and convenient operation.

Active Publication Date: 2018-09-11
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology

Although the morphological method is simple and intuitive, it is easily affected by the environment and has low reproducibility; it is difficult to classify genotypes with very close traits by phenotypic observation

Method used

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  • InDel labeling primer pair used for distinguishing five capsicum cultispecies and application thereof
  • InDel labeling primer pair used for distinguishing five capsicum cultispecies and application thereof
  • InDel labeling primer pair used for distinguishing five capsicum cultispecies and application thereof

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Embodiment Construction

[0022] 1. Test materials

[0023] The two re-sequenced materials include G29, which belongs to Capsicum annuum, which is a high-generation inbred line material, and comes from the Vegetable Research Institute of Jiangsu Academy of Agricultural Sciences; PBC688, which belongs to Capsicum frutescens, is introduced from the Asian Vegetable Center. The 55 pepper materials used to verify the accuracy of the labeling are all derived from the National Plant Germplasm System (NPGS), which belong to 5 pepper cultivars. See Table 1 for details.

[0024] Table 1. 55 materials used to verify marking accuracy

[0025]

[0026]

[0027] 2. InDel mark development

[0028] Use the resequencing data to compare with the reference genome to find the InDel site. The reference genome version is pepper.V.1.55, and the download URL is http: / / peppergenome.snu.ac.kr / download.php. The illuminaHiSeqTM2500 sequencing platform was used to sequence annual pepper (Capsicum annuum) "G29" and shrub pepper (Capsicum...

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Abstract

The invention discloses an InDel labeling primer pair used for distinguishing five capsicum cultispecies and application thereof. The InDel labeling primer pair is composed of a forward primer which is shown in SEQ ID NO.1 and a reverse primer which is shown in SEQ ID NO.2. Genome DNAs of the five capsicum cultispecies are utilized to conduct PCR amplification. If a 133bp molecular marker spectrumband exists, it is shown that the capsicum belongs to Capsicum annuum; if a 135bp molecular marker spectrum band exists, it is shown that the capsicum belongs to Capsicum frutescens; if a 138bp molecular marker spectrum band exists, it is shown that the capsicum belongs to Capsicum Chinese; if a 141bp molecular marker spectrum band exists, it is shown that the capsicum belongs to Capsicum baccatum; if a 148bp molecular marker spectrum band exists, it is shown that the capsicum belongs to Capsicum Pubescens. The marker can be used for distinguishing the capsicum cultispecies and hybrids thereof, a molecule basis is provided for the division of the capsicum cultispecies, and a more reliable and accurate identification method is provided for identification of distant hybrids.

Description

Technical field [0001] The invention relates to an InDel marker primer pair for distinguishing five pepper cultivars and its application, and belongs to the field of agricultural biotechnology engineering. technical background [0002] Pepper is one of the very important vegetable crops in Solanaceae, which originated in Central and South America. As peppers have both the functions of vegetables and condiments, they have extremely high economic value worldwide. 31 species have been identified in the genus Capsicum, including five cultivars of pepper, namely: annual pepper (Capsicum annuum L.), Chinese pepper (Capsicumchinense Jacq.), shrub-like pepper (Capsicum frutescens L.), and drooping pepper (Capsicum baccatumL.) and pilose pepper (Capsicum pubesens Ruiz&Pavon). There are morphological differences in many traits among the five cultivars, such as the most obvious flower and seed color. [0003] Traditional plant classification is based on the morphological characteristics of...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 郭广君王述彬潘宝贵刘金兵刁卫平戈伟高长洲
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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