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Tissue culture method and medium used for direct seedling growth of Zizania zaziza officinalis seed embryo

A technique of tissue culture medium and tissue culture, which is applied in the field of plant tissue culture, can solve the problems of poor character stability of Zizania zizania and easy variation of ramification propagation of Zizania zizania, and achieve the effect of high tissue culture efficiency and reduced time

Active Publication Date: 2021-06-18
ZHEJIANG NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the interaction with smut fungus to conceive Zizania, the existing Zizania ramets tend to mutate, so the stability of Zizania is poor.

Method used

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  • Tissue culture method and medium used for direct seedling growth of Zizania zaziza officinalis seed embryo

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1, Zizania zizania seed embryo in vitro culture medium (that is, seedling growth medium), its preparation method is as follows:

[0035] ①, configure the mother liquor of plant growth regulating substances:

[0036] 6-benzyladenine (6-BA), naphthaleneacetic acid (NAA), and kinetin (KT) as plant growth regulators were treated as follows:

[0037] After weighing 1 mg of plant growth regulator substance and dissolving it with 95% (volume %) ethanol (the amount of the ethanol only needs to make the plant growth regulator substance dissolve), add distilled water and carry out constant volume to 5ml, thereby respectively making 0.2mg / ml ml of 6-benzyladenine in water, 0.2 mg / ml of naphthalene acetic acid in water, and 0.2 mg / ml of kinetin in water.

[0038] The above three aqueous solutions were respectively sterilized by filtration through a 0.2 μm microporous membrane to obtain 6-BA mother solution (0.2 mg / ml), NAA mother solution (0.2 mg / ml) and KT mother solut...

Embodiment 2

[0042] Embodiment 2, a method for in vitro culture of Zizania seed embryos, using the seedling growth medium prepared in Example 1, taking plump newly harvested Zizania seeds as experimental materials and proceeding as follows:

[0043] 1) Select plump Zizania zizania seeds, use sterilized tweezers to cut off the exposed embryos at 1 / 4 of the blunt end of the sterilized seeds, obtain seed embryos, and inoculate the seed embryos on the seedling medium .

[0044] The Zizania seeds used in this example are the seeds of Jinjiao No. 1 male plant collected from the Zizania Base in Jinhua City.

[0045] Note: Zizania zizania seeds are thin and long, one end of the seed is blunt and round, and the other end (the awn end) protrudes, and the seed embryo is close to the end of the blunt round seed.

[0046] The above-mentioned method for disinfecting and sterilizing the Zizania seed is as follows:

[0047]Select plump Zizania seeds, peel off the shell, and then wash the above-mentioned...

Embodiment 3

[0050] Embodiment 3, the Zizania seed of choosing in embodiment 2 is changed into " the male plant seed of Zhejiao No. 3 (double-season Zizania variety) " by " the male plant seed of Jinjiao No. 1 (single-season Zizania variety) ", the rest Identical to embodiment 2. A total of 180 immature embryos were inoculated (ie, 3 repetitions were set up, 60 embryos were inoculated each time), and the seedling rate (mean value) was 80%.

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Abstract

The invention discloses a method for preparing a medium for direct seedling growth of Zizania zizania seed embryos. Take 1LMS basic culture solution and adjust the pH value to 5.7-5.9, then add 2.5-4.2g plant gel and 28-32g sucrose, and sterilize at high temperature When the temperature of the sterilized solution drops to 50-60° C., add 2.0 mg of 6-BA mother solution, 2.0 mg of KT mother solution, and 0.2 mg of NAA mother solution to obtain a seedling growth medium. The invention also discloses a method for direct seedling tissue culture of Zizania zizania seed embryos: selecting Zizania zizania seeds for disinfection and sterilization to obtain the seed embryos; inoculating the seed embryos on the seedling growth medium, and performing light irradiation at a temperature of 22-27°C For cultivation, the light intensity is 40‑60 μmol / m 2 / s, the daily light time is 16 hours, until 2 to 3 leaves grow, and green seedlings are obtained, which can significantly reduce the time from mature seeds to seedlings of Zizania zalea.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for directly growing seedling tissue culture of Zizania zizania seed embryos and the culture medium used. Background technique [0002] Zizania caducifloro Hand-Mozz [Z. LatifliaTurcz], also known as Zizania caducifloro Hand-Mozz [Z. Latiflia Turcz], belongs to Gramineae and is a perennial aquatic perennial herb with a plant height of about 2m and no taproot. Many adventitious roots emerge from the stem nodes. The wild rice stems planted in our country only grow leaves and stems, and do not flower and set seeds, while wild rice is the one that blooms and sets seeds. Compared with "five grains", wild rice has inconsistent grain maturity, the seeds are easy to fall off, harvesting is difficult, the yield is low, and it also affects the growth of bamboo shoots. Once farmers find it, they immediately pull it out. Therefore, only smut is planted in our country now. Parasi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01H6/46
CPCA01H4/001A01H4/005A01H4/008A01H5/00
Inventor 查笑君柴飘飘马伯军张尚法杨梦飞郑寨生曹春信王凌云李怡鹏
Owner ZHEJIANG NORMAL UNIVERSITY
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