Kit for rapidly detecting sperm activity and detection method

Abstract

The invention discloses a kit for rapidly detecting sperm activity, the kit comprises a colloidal gold test strip and a buffer for dilution of semen, wherein a mouse anti-human TEX101 protein antibodyis immobilized on a T test zone membrane of the colloidal gold test strip, and another paired mouse anti-human TEX101 protein antibody is immobilized on a C quality control zone membrane of the colloidal gold test strip. The kit for rapidly detecting the sperm activity can quickly determine whether symptoms are oligospermia or azoospermia by detecting the protein concentration of TEX101 in the human semen.

Description

technical field [0001] The invention relates to a kit for rapidly detecting sperm activity, which adopts colloidal gold immunochromatography analysis technology and double-antibody sandwich method to qualitatively detect the protein concentration of TEX101 in human semen, belonging to the field of in vitro diagnostic reagents. Background technique [0002] In recent decades, with the influence of factors such as environmental pollution, bad living habits and the spread of sexually transmitted diseases, human fertility has declined day by day. Worldwide, human male sperm count and sperm volume have each declined by 50% over the past 50 years. According to the standards of the World Health Organization, couples who live together for more than one year after marriage without taking any contraceptive measures are called infertile. Among them, those who are caused by the man's cause of female infertility are called male infertility. 50% of the entire sterility factor. In 2012, ...

AI Technical Summary

Problems solved by technology

However, this method is to use the puncture method to obtain the specimen, which will cause high cost, and may cause secondary or multiple sampling and invasive defects due to the influence of the puncture site.