Transcription factor pdd1, its coding gene and application that regulates the development of fruiting bodies of Flammulina velutipes

Abstract

The invention discloses a transcription factor PDD1 for regulating development of fruiting body of flammulina velutipes and an encoding gene and an application thereof. The coding gene sequence of thetranscription factor PDD1 is shown in the sequence 1 in the sequence table; the amino acid sequence of the transcription factor is shown in sequence 2 in the sequence table. According to the method,the overexpression of the pdd1 and the RNAi experiment in the flammulina velutipes are carried out, and the pdd1 is a positive control factor which is necessary during the growth and the fruiting bodydevelopment of the flammulina velutipes, and influences the original basal period and the maturity of the flammulina velutipes, the number of the fruiting bodies, the height and the wet weight. The invention also discloses a flammulina velutipes mutant strain (pdd1OE#31) with short fruiting period and high yield, which not only has the characteristics of early maturity, but also can improve the yield of the flammulina velutipes, shorten the production cycle of the flammulina velutipes, reduce the energy consumption and the labor cost, and has great application prospect.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a transcription factor PDD1 that positively regulates the mycelial growth and fruiting body development of Flammulina velutipes, and a pdd1 overexpression mutant strain (pdd1) with short fruiting period and high yield. OE#31 ). Background technique [0002] Flammulina velutipes (Flammulina velutipes), also known as mushrooms, mushrooms, and money mushrooms, is a typical basidiomycete edible fungus, which is widely cultivated in the world, especially in Asian countries. Flammulina velutipes is rich in protein, dietary fiber and carbohydrates, and also rich in vitamins B1, B2, B3 and vitamins C, D, and E. As a traditional edible fungus, Flammulina velutipes not only has rich nutritional value, but also has important medicinal value. Studies in recent years have shown that Flammulina velutipes has anti-tumor, prolong life, lower cholesterol, anti-fatigue, immune regula...

AI Technical Summary

Problems solved by technology

For example, fvh1 is highly expressed during the development of fruiting bodies of Flammulina velutipes, and the overexpression of the hydrophobin-encoding gene Fv-hyd1 advances the formation of primordium, histidine kinase gene and GATA Zinc finger transcription factor participate in the process of cold-induced primordium formation, Mads8, The HK535 gene was differentially expressed in primordia and hyphae. Knocking down the adenosine deaminase encoding gene Fv-ada could inhibit the mycelial growth of Flammulina velutipes. The analysis of the mating type genes of Flammulina velutipes, the laccase-encoding gene is involved in the formation of the fruiting body of Flammulina velutipes, and the overexpression of Fv-JRL1 promotes the mycelial growth and fruiting body development of Flammulina velutipes, but these studies are limited to the study of gene structure or gene function. The application in Flammulina velutipes has yet to be developed

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