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Molecular markers and their application of the main effect qtl of Zhenwei rice storage tolerance

A molecular marker and storage-resistant technology, applied in the field of agricultural biology, can solve problems such as consumption, poor germination rate, and commercial decline, and achieve the effects of accelerated screening efficiency, stable amplification, and high accuracy

Active Publication Date: 2021-10-22
福建六三种业有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The national grain reserve adopts the method of dispersing and batching, regularly updating the old and storing the new, and taking turns to update, but it will consume a lot of manpower, material resources and financial resources; the storage of rice will be affected by the decline in quality and commerciality due to the decline in quality, mildew, and storage pests. Seriously threaten food security and cause major economic losses to the country; similarly, seed reserves also reduce seed viability and germination rate due to aging and other reasons, which often have a certain impact on production
For ordinary farmers, although it is possible to delay the aging rate of rice during storage by regularly pushing old and storing fresh or by improving storage conditions, such as building controlled atmosphere storage or low-temperature storage, these methods not only consume a lot of energy and materials, And it is not suitable for the majority of farmers to adopt

Method used

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  • Molecular markers and their application of the main effect qtl of Zhenwei rice storage tolerance

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] At the beginning of November 2014, we selected 30 newly harvested rice varieties with significant differences in storage performance in the years of production practice in the field of the year, and tested the germination rate after drying to break dormancy, and selected 14 rice varieties with germination rates greater than 92%. The varieties were subjected to natural aging tests, and the seed germination rates of 14 varieties under natural aging were identified and counted on November 8, 2015. In December 2015, the newly harvested seeds of these 14 rice varieties were selected for germination rate identification after drying and breaking dormancy. It was found that the germination rate was greater than 90%, and there was no significant difference in germination rate among varieties. The seeds with full grains and no damage were screened in the experiment. The artificial aging method of the present invention and the two artificial aging methods (chemical method and high-...

Embodiment 2

[0026] The main effect QTL for storage tolerance of Zhenwei rice wxya The molecular marker localization steps are as follows.

[0027] (1) Build a targeting group

[0028] Using the excellent storage-tolerant rice variety Zhenweidao as the female parent and the non-storage-resistant rice variety Xudao No. 3 as the male parent, a hybrid F1 was prepared, and an F2 segregation population containing 228 individuals was constructed. F2 individual plants were self-crossed to obtain F2: 3 families.

[0029] (2) Identification of storability phenotypes of parent and offspring of F2:3 family

[0030] The improved seed artificial aging method was used to process the parent and offspring materials of the F2:3 family. After the treatment was completed, the germination rate of the seeds was detected, and the storage resistance of the seeds was graded according to the difference in the germination rate. The rice material with a germination rate of 0 was marked as 0. Rice materials with ...

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Abstract

The invention discloses a molecular marker and an application of the main effect QTL for storage tolerance of rare rice, and belongs to the field of biotechnology. Firstly, the main effect QTL related to the storage tolerance of Zhenwei rice was disclosed wxya The closely linked molecular marker RM211, and the sequence of the marker's primer pair are also disclosed. Containing QTL wxya In the rice material of , the product of about 144bp can be amplified by using the molecular marker RM211 primer pair. The present invention discloses and wxya Tightly linked molecular markers can be used for molecular marker-assisted selection breeding of rice for storage tolerance.

Description

technical field [0001] The invention discloses a molecular marker and an application of the main effect QTL for storage resistance of delicacy rice, and belongs to the field of agricultural biotechnology. Background technique [0002] Rice ( Oryza sativa L.) is one of the most important food crops in the world, and also one of the most important food crops in my country. my country is an ancient country of rice production and the largest rice producer in the world. It is extremely important to my country's economic development and people's life significance. At present, among the three major food crops in my country, rice, wheat, and corn, rice ranks first in terms of sowing area and output. The annual planting area of ​​rice in my country is about 30 million hectares, accounting for 30% of the national grain planting area and 20% of the world's rice planting area. %, the average yield of rice is 6.212 tons per hectare, which is the grain crop with the highest yield per un...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 隋福权段庆松杜雪
Owner 福建六三种业有限责任公司