A kind of carotenoid-albumin complex and its preparation method and application
A technology of carotene and albumin, which is applied in the direction of effective components of hydrocarbon compounds, application, effective components of hydroxyl compounds, etc., can solve the problems of low drug loading and encapsulation efficiency, poor chemical stability of emulsions, complex preparation process, etc., to achieve Good application effects, wide range of applications, and effects from a wide range of sources
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Embodiment 1
[0029] Preparation of an astaxanthin-bovine serum albumin complex
[0030] Weigh 200mg bovine serum albumin and dissolve it in 50ml deionized water, and weigh 10mg astaxanthin and dissolve it in 10ml chloroform at the same time, take the aqueous phase and the organic phase according to the volume ratio of 5:1, and place the two in a microjet high-pressure homogenization In the machine, circulate 10 times under the pressure of 1200bar until the mixture is uniform, and then remove the organic solvent in the solution by rotary evaporation under reduced pressure at 40°C to obtain a transparent astaxanthin-bovine serum albumin complex solution. The average particle diameter is 96nm.
Embodiment 2
[0032] Preparation of a β-carotene-human serum albumin complex
[0033] Weigh 150mg of human serum albumin and dissolve it in 60ml of deionized water, and weigh 10mg of β-carotene and dissolve it in 6ml of dichloromethane at the same time, take the aqueous phase and the organic phase according to the volume ratio of 10:1, and place the two in an ultrasonic breaker , ultrasonicated 20 times at 600W until evenly mixed, and then rotated under reduced pressure at 40°C to remove the organic solvent in the solution to obtain a clear β-carotene-human serum albumin complex solution. The average The particle size is 158nm.
Embodiment 3
[0035] Preparation of a Lycopene-Recombinant Human Serum Albumin Complex
[0036] Weigh 300mg of recombinant human serum albumin and dissolve it in 90ml of deionized water, and at the same time weigh 10mg of lycopene and dissolve it in 5ml of ethyl acetate. Take the aqueous phase and the organic phase according to the volume ratio of 18:1, and place the two under high pressure. In the quality machine, circulate 15 times under the pressure of 1100bar until the mixture is uniform, and then remove the organic solvent in the solution by rotary evaporation under reduced pressure at 40°C to obtain a clear lycopene-recombinant human serum albumin complex solution. The average particle size obtained was 136nm. The solution is placed in a freeze dryer and freeze-dried to obtain a freeze-dried powder.
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