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Complex microbial inoculant containing bacillus subtillis B2-3-2 and bacillus subtillis B19 and applications of complex microbial inoculant

A Bacillus subtilis and composite microbial agent technology, applied in the field of Bacillus subtilis B2-3-2 and Bacillus subtilis B19 composite bacterial agent, can solve problems that have not yet been discovered

Active Publication Date: 2018-09-28
XIAN DELONG BIOSCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that the strains producing chitinase and serine protease have been used in agricultural microbial preparations respectively, but in the published reports and patents, it has not been found that two strains of Bacillus subtilis producing chitinase and serine protease can be combined Reports and patents on the production of agricultural microbial agents and fertilizers

Method used

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  • Complex microbial inoculant containing bacillus subtillis B2-3-2 and bacillus subtillis B19 and applications of complex microbial inoculant
  • Complex microbial inoculant containing bacillus subtillis B2-3-2 and bacillus subtillis B19 and applications of complex microbial inoculant
  • Complex microbial inoculant containing bacillus subtillis B2-3-2 and bacillus subtillis B19 and applications of complex microbial inoculant

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Experimental program
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Effect test

Embodiment 1

[0038] The composite bacterial agent containing Bacillus subtilis B2-3-2 and Bacillus subtilis B19 of the present embodiment, Bacillus subtilis (Bacillus subtilis) B2-3-2, was preserved in Beijing on December 21, 2017 in Chinese microbial strains The General Microorganism Center of the Preservation Management Committee, the preservation number CGMCC No.15114, Bacillus subtilis (Bacillus subtilis) B19, was deposited in the General Microbiology Center of the China Committee for the Collection of Microorganisms on December 21, 2017, and the preservation number was CGMCC No.15113 . The compound bacterial agent is prepared by conventional methods in liquid form from Bacillus subtilis B2-3-2 and Bacillus subtilis B19, and in 1mL of liquid compound bacterial agent, it contains 4.8~5.2×10 8 cfu Bacillus subtilis B2-3-2, containing 4.8~5.2×10 8 cfu Bacillus subtilis B19. In 1mL of liquid complex microbial agent, it is preferable to contain 4.9~5.1×10 8 cfu Bacillus subtilis B2-3-2, ...

Embodiment 2

[0058] The composite microbial agent containing Bacillus subtilis B2-3-2 and Bacillus subtilis B19 of the present embodiment is prepared by mixing Bacillus subtilis B2-3-2 and Bacillus subtilis B19 in a solid form according to a conventional method, and it is prepared in 1 g In the solid compound bacterial agent, it contains 2.48~2.52×10 9 cfu Bacillus subtilis B2-3-2, containing 2.48~2.52×10 9 cfu Bacillus subtilis B19. In 1g of solid composite microbial agent, preferably contain 2.49~2.51×10 9 cfu Bacillus subtilis B2-3-2, 2.49~2.51×10 9 cfu Bacillus subtilis B19.

[0059] Take the production of 1000kg Bacillus subtilis B2-3-2 and Bacillus subtilis B19 solid composite microbial agent as an example, its preparation method is as follows:

[0060] 1. Preparation of slant strains

[0061]Weigh 0.3g of beef extract, 1g of peptone, and 0.5g of sodium chloride, dissolve them in 100mL of distilled water, heat to boiling, add 2g of agar, heat to completely melt the agar, and adj...

Embodiment 3

[0078] The liquid composite bacterial agent containing bacillus subtilis B2-3-2 and bacillus subtilis B19 of embodiment 1 and the solid composite bacterial agent containing bacillus subtilis B2-3-2 and bacillus subtilis B19 of embodiment 2 are used for anti- Crop diseases caused by Fusarium oxysporum.

[0079] When in use, add 2L of liquid compound bacterial agent containing Bacillus subtilis B2-3-2 and Bacillus subtilis B19 into 500L water, 2 (1 mu) Vegetable roots in the vegetable field can also be used to add 2L of liquid compound bacterial agent containing Bacillus subtilis B2-3-2 and Bacillus subtilis B19 to 2000L water, and use drip irrigation to drip into the roots of vegetables. . Add 2kg of solid composite bacterial agent containing Bacillus subtilis B2-3-2 and Bacillus subtilis B19 into 500L water, 2 (1 mu) Vegetable roots in the vegetable field can also be irrigated by adding 2kg of solid composite bacterial agent containing Bacillus subtilis B2-3-2 and Bacillus s...

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Abstract

The invention provides a complex microbial inoculant containing bacillus subtillis B2-3-2 and bacillus subtillis B19 and applications of the complex microbial inoculant. The bacillus subtillis B2-3-2is preserved in the China General Microbiological Culture Collection Center in Beijing on Dec 21, 2017, and is assigned with the accession number of CGMCC No.15114, the bacillus subtillis B19 is preserved in the China General Microbiological Culture Collection Center in Beijing on Dec 21, 2017, and is assigned with the accession number of CGMCC No.15113, the complex microbial inoculant is preparedby mixing according to the conventional method in the form of liquid or solid, wherein 1mL of the liquid complex microbial inoculant contains (4.8-5.2)x10<8> cfu bacillus subtillis B2-3-2 and (4.8-5.2)x10<8> cfu bacillus subtillis B19, and 1g of the solid complex microbial inoculant contains (2.48-2.52)x10<9> cfu bacillus subtillis B2-3-2 and (2.48-2.52)x10<9> cfu bacillus subtillis B19.

Description

technical field [0001] The invention belongs to the technical field of microbial fertilizers, and in particular relates to a composite microbial agent of bacillus subtilis B2-3-2 and bacillus subtilis B19. Background technique [0002] With the reduction of arable land and the development of protected cultivation, continuous cropping is inevitable. However, the ensuing obstacles to continuous cropping have resulted in a large area of ​​crop yield reduction or even failure. Soil-borne fungal diseases and root-knot nematode damage are important reasons for crop continuous cropping obstacles, and biological control has great potential in controlling plant soil-borne fungal diseases and root-knot nematode damage. [0003] Microbial fertilizers, referred to as bacterial fertilizers, refer to products containing specific living microorganisms, which are used in agricultural production. Through the life activities of microorganisms contained in them, they can increase the supply o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P5/00C12R1/125
CPCA01N63/00C12N1/20C12N1/205C12R2001/125
Inventor 陶树兴芦晓飞凌键刘甲启孙宏斌张万里郝小武田星
Owner XIAN DELONG BIOSCI