Method for preparing growth factor controllable artificial tissue and organ soft scaffold

Abstract

The invention provides a method for preparing a growth factor controllable artificial tissue and organ soft scaffold. The method comprises the following steps: modeling by virtue of computer aided design, and designing and controlling scaffold shape, the inner structure, size, porosity and the like; taking a hydrogel material as a biological scaffold, wherein the hydrogel has a high polymer porousnetwork system, is favorable for cell adhesion and growth and proliferation, soft in property and high in plasticity, and is conveniently processed to obtain needed tissue or organ shapes; in the printing process, printing a layer of hydrogel matrix material by adopting a manner of cooperatively printing by multiple nozzles for the whole scaffold, printing a histiocyte layer on the scaffold, printing growth factors according to a certain concentration gradient distribution requirement after cross-linking, realizing layered superposition of the hydrogel material, cells and growth factors, andfinally printing the controllable three-dimensional soft scaffold structure meeting the concentration gradient of the growth factors. The activities of the growth factors and cells can be ensured, andthe accuracy and controllability of the concentration gradient distribution of the growth factors inside the soft tissue scaffold can be realized.

Description

technical field [0001] The invention relates to the technical field of biological tissue engineering, in particular to a method for preparing a growth factor-controllable artificial tissue and organ soft support. Background technique [0002] At present, the clinical cases of organ failure or tissue damage caused by disease, aging, accidents, wars, etc. are increasing year by year. In medicine, organ transplantation, artificial substitutes and drugs are often used to treat tissue and organ lesions; Organs are in short supply, and immune rejection is prone to occur during transplantation. Patients must use anti-immune drugs for a long time, so the success rate of transplantation is relatively low. The structure of the artificial substitute scaffold is controllable and has good biocompatibility, which is beneficial to the cultivation of living tissues and organs in vitro and increases the possibility of transplantation. [0003] Human tissues and organs belong to soft tissue,...

AI Technical Summary

Problems solved by technology

This patent uses living tissue cells and gel to prepare the scaffold, and can effectively control the microstructure and shape of the soft scaffold, but this method only prints a mixture of cells and gel, and cannot meet the requirements for the precise distribution of growth factors inside the soft scaffold