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A method for treating coal mine wastewater by sulfate-reducing bacteria in cooperation with spontaneously combusted coal gangue

A technology of coal mine wastewater and sulfate, applied in mining wastewater treatment, chemical instruments and methods, biological water/sewage treatment, etc., to achieve the effect of improving the polluted environment, obvious adsorption effect, and thorough treatment

Active Publication Date: 2020-06-02
LIAONING TECHNICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the chemical method and the constructed wetland method, the object of the present invention is to provide a method for treating coal mine wastewater with sulfate-reducing bacteria in cooperation with spontaneous combustion coal gangue, especially for treating Fe-containing waste water. 2+ , Mn 2+ and SO 4 2- Static test method for coal mine wastewater

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A method for treating coal mine wastewater by sulfate-reducing bacteria in cooperation with spontaneous combustion coal gangue, comprising the following steps:

[0038] Step 1: Preparation of spontaneous combustion gangue powder

[0039] Sieve the spontaneous combustion coal gangue samples taken from Gaode Mine in Fuxin, Liaoning, to obtain spontaneous combustion coal gangue powder with a particle size of 0.074-0.600mm, dip in distilled water for 3 times, and dry at 60°C to obtain dry spontaneous combustion coal Gangue powder, spare;

[0040] Step 2: Preparation of sulfate-reducing bacteria

[0041] Using the bottom mud of Xihe River in Fuxin City as activated sludge, under anaerobic environment, using Postgate medium, using liquid paraffin liquid seal, rubber stopper to stopper bottle mouth and Vaseline seal, after enrichment culture, the medium turns black, Open the cork to smell the hydrogen sulfide gas, and get the SRB strains in the logarithmic growth phase;

[00...

Embodiment 2

[0058] A method for treating coal mine wastewater with sulfate-reducing bacteria in cooperation with spontaneously combusted coal gangue, the same as in Example 1.

[0059] The difference is that

[0060] 1) In step 1, the spontaneous combustion coal gangue powder with a particle size of 0.600 to 0.450 mm is obtained;

[0061] 2) In step 3, dry spontaneous combustion coal gangue powder: SRB bacteria suspension after centrifugation: Fe-free 2+ Postgate Medium = 30g: 5mL: 100mL;

[0062] Others are the same.

[0063] The method for treating coal mine wastewater by using the sulfate-reducing bacteria of this embodiment in cooperation with spontaneously combusted coal gangue can remove Fe 2+ , Mn 2+ and SO 4 2- , where Fe 2+ The removal rate was 70.94%, Mn 2+ The removal rate was 83.33%, SO 4 2- The removal rate is 65.40%, and after treatment, the pH value of coal mine wastewater is 6.91.

Embodiment 3

[0065] A method for treating coal mine wastewater with sulfate-reducing bacteria in cooperation with spontaneously combusted coal gangue, the same as in Example 1.

[0066] The difference is that

[0067] 1) In step 1, the spontaneous combustion coal gangue powder with a particle size of 0.450 to 0.300 mm is obtained;

[0068] 2) In step 3, dry spontaneous combustion coal gangue powder: SRB bacteria suspension after centrifugation: Fe-free 2+ Postgate medium = 30g: 10mL: 100mL;

[0069] Others are the same.

[0070] The method for treating coal mine wastewater by using the sulfate-reducing bacteria of this embodiment in cooperation with spontaneously combusted coal gangue can remove Fe 2+ , Mn 2+ and SO 4 2- , where Fe 2+ The removal rate was 84.38%, Mn 2+ The removal rate was 65.48%, SO 4 2- The removal rate is 77.58%. At the same time, the pH value of coal mine wastewater is 7.51 after treatment.

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Abstract

A method of treating coal mine wastewater by synergy of sulfate-reducing bacteria and spontaneous combustion gangue belongs to the field of wastewater treatment and comprises: screening spontaneous combustion gangue samples to obtain spontaneous combustion gangue powder having particle size of 0.074-0.600 mm, and drying; with wastewater sediment as activated sludge, performing enrichment culture with Postgate medium in an anaerobic environment to obtain an SRB (sulfate-reducing bacteria) strain in logarithmic phase, and centrifuging to obtain an SRB suspension; adding the spontaneous combustion gangue powder and the SRB suspension into Postgate medium having no Fe2+, sealing the liquid, culturing in a thermostatic vibrating culture tank at 30-35 DEG C for 5-7 days to obtain a sulfate-reducing bacteria and spontaneous combustion gangue sample, adding the sulfate-reducing bacteria and spontaneous combustion gangue sample into coal mine wastewater at 30-35 DEG C, and performing wastewatertreatment. The method that treats coal mine wastewater by synergy of microorganisms and spontaneous combustion gangue can help reduce environment pollution due to coal mine wastewater and solve the coal gangue piling problem and has a good application prospect.

Description

technical field [0001] The invention relates to the field of wastewater treatment, in particular to a method for treating coal mine wastewater by sulfate-reducing bacteria in cooperation with spontaneously combusted coal gangue. Background technique [0002] Coal mine wastewater is formed by the leaching of sulfur and heavy metal elements in the long-term rainwater during the mineral mining process. The highly acidic and heavy metal-rich coal mine wastewater is discharged directly without treatment, which will seriously pollute the water and soil in the mining area. The pollution of coal mine wastewater is a global problem. At present, the methods of treating coal mine wastewater at home and abroad mainly include chemical methods, constructed wetland methods and microbial methods. Among them, the chemical method includes neutralization method, ion exchange method, membrane separation method, etc.; the constructed wetland method uses the joint action of artificial medium, soi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34C02F103/10
CPCC02F3/345C02F2103/10
Inventor 郭旭颖里莹刘威狄军贞董艳荣邢经纬付赛欧
Owner LIAONING TECHNICAL UNIVERSITY
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