A method for improving the germination rate of free microspores of alfalfa

A technology of spore germination rate and alfalfa, which is applied in the field of biological sciences, can solve the problems of low germination rate and achieve the effects of increasing embryonic rate, improving selection efficiency, and shortening the breeding cycle

Active Publication Date: 2022-01-21
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

[0004] In order to solve the technical problem in the prior art that alfalfa adopts free microspores to culture and the embryo emergence rate is low, the present invention provides a method for improving the embryo emergence rate of alfalfa free microspores, the purpose of which is to be able to quickly and efficiently obtain The embryonic rate of microspore embryos of alfalfa reaches 44.22 per bud, and the regenerated plants obtained can be used for haploid breeding of alfalfa

Method used

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  • A method for improving the germination rate of free microspores of alfalfa
  • A method for improving the germination rate of free microspores of alfalfa

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Embodiment 1

[0016] Embodiment one: a kind of method that the present invention improves the free microspore germination rate of alfalfa, the method comprises in the blooming stage of alfalfa, from the donor plant, selects robust flower buds without damage by diseases and insect pests, selects free microspores to be in the mononuclear habitat The step of cultivating flower buds in the middle stage. During the blooming period of alfalfa, the time to extract healthy and pest-free flower buds from the donor plant is to extract from the donor plant at 8:00-10:00 in the morning on a sunny day.

[0017] Show through part of experiment below, the present invention selects free microspore to be in the flower bud of uninucleate middle stage and cultivates and can improve microspore embryo rate, has compared the influence of the microspore development period on the free microspore embryo rate of alfalfa, including single The results showed that the embryonic rate of free microspores at the single-nu...

Embodiment 2

[0020] Embodiment two: a kind of method that the present invention improves the free microspore germination rate of alfalfa, the method comprises in the blooming stage of alfalfa, from the donor plant, selects robust and pest-free flower buds, and selects free microspores to be in the mononuclear habitat The step of cultivating flower buds in the middle stage. During the blooming period of alfalfa, the time to extract healthy and pest-free flower buds from the donor plant is to extract from the donor plant at 8:00-10:00 in the morning on a sunny day.

[0021] Further, before culturing the flower buds, the flower buds were first sterilized with 75% alcohol for 30 seconds, then sterilized with 8% sodium hypochlorite for 12 minutes, rinsed with sterile water for 5 times, and then placed in a sterile mortar.

[0022] The culture method comprises adding half an amount of MS liquid medium and B5 liquid medium in a sterile mortar, the volume ratio of MS liquid medium and B5 liquid me...

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Abstract

The invention discloses a method for increasing the germination rate of free microspores of alfalfa, which comprises selecting healthy and pest-free flower buds from donor plants at the full-blooming stage of alfalfa, and selecting flower buds with free microspores in the uninucleated middle stage for cultivation. The invention has the advantages that microspore embryos of alfalfa can be obtained quickly and efficiently, and the embryo emergence rate reaches 44.22 per bud, and the obtained regenerated plants can be used for haploid breeding of alfalfa.

Description

technical field [0001] The invention belongs to the technical field of biological sciences, in particular to a method for improving the germination rate of free microspores of alfalfa. Background technique [0002] Haploid breeding has great commercial application value. Using haploid induction to produce haploid plants, and then doubling naturally or artificially, homozygous breeding materials can be obtained within two years, which can significantly speed up the selection process and shorten the breeding cycle. Alfalfa (Medicago sativa L.) is a perennial cross-pollinated plant, the genetic composition of its population is complex and diverse, the genotype is in a heterozygous state for a long time, and the plants are self-incompatible. Breeding new varieties by means of recurrent selection. However, it takes at least 6-7 years to breed an excellent population using the above method, and it is extremely difficult to obtain homozygous materials. The breeding cycle is long, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/005
Inventor 仪登霞王赞苏彦宾
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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