Sturgeon SNP (single nucleotide polymorphism) molecular markers, sturgeon seed production evaluation method and genetic evaluation method
A molecular marker and sturgeon technology, which is applied in the field of sturgeon SNP molecular markers and genetic evaluation, can solve the problems of genetic diversity analysis, breeding problems of improved species, scarcity of microsatellite markers, and difficulties in nuclear gene analysis and research. Broad range, avoidance of cumbersomeness, and cost reduction effects
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[0061] Genetic Evaluation of 18 Candidate Parents of Chinese Sturgeon
[0062] 1. Genomic DNA Extraction
[0063] Blood was collected from 18 candidate broodstocks of Chinese sturgeon, including 12 females (A01-A12) and 6 males (B01-B06). Blood was anticoagulated with EDTA and stored at 4°C. Take 20 μL of blood, use the Marine Animal Genomic DNA Extraction Kit (Tiangen DP324-03), extract genomic DNA according to the instructions, dissolve it in 100 μL of sterilized deionized water, and measure the concentration with an ultraviolet spectrophotometer.
[0064] 2. SNP typing
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