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Sturgeon SNP (single nucleotide polymorphism) molecular markers, sturgeon seed production evaluation method and genetic evaluation method

A molecular marker and sturgeon technology, which is applied in the field of sturgeon SNP molecular markers and genetic evaluation, can solve the problems of genetic diversity analysis, breeding problems of improved species, scarcity of microsatellite markers, and difficulties in nuclear gene analysis and research. Broad range, avoidance of cumbersomeness, and cost reduction effects

Active Publication Date: 2018-10-16
CHINESE ACAD OF FISHERY SCI
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Problems solved by technology

However, due to the polyploidy of the sturgeon genome and the ploidy differences among different species, it brings difficulties to the analysis of nuclear genes, and the number of microsatellite markers developed in sturgeon species is also relatively rare compared with other model aquatic organisms , causing great troubles to vigorously develop sturgeon molecular markers, analysis of sturgeon genetic diversity, and breeding of improved species

Method used

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  • Sturgeon SNP (single nucleotide polymorphism) molecular markers, sturgeon seed production evaluation method and genetic evaluation method
  • Sturgeon SNP (single nucleotide polymorphism) molecular markers, sturgeon seed production evaluation method and genetic evaluation method
  • Sturgeon SNP (single nucleotide polymorphism) molecular markers, sturgeon seed production evaluation method and genetic evaluation method

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Embodiment 1

[0061] Genetic Evaluation of 18 Candidate Parents of Chinese Sturgeon

[0062] 1. Genomic DNA Extraction

[0063] Blood was collected from 18 candidate broodstocks of Chinese sturgeon, including 12 females (A01-A12) and 6 males (B01-B06). Blood was anticoagulated with EDTA and stored at 4°C. Take 20 μL of blood, use the Marine Animal Genomic DNA Extraction Kit (Tiangen DP324-03), extract genomic DNA according to the instructions, dissolve it in 100 μL of sterilized deionized water, and measure the concentration with an ultraviolet spectrophotometer.

[0064] 2. SNP typing

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Abstract

The invention provides sturgeon SNP (single nucleotide polymorphism) molecular markers. The invention further provides a method of sturgeon candidate parent genetic evaluation. The invention further provides an evaluation method of a seed production parent in a sturgeon candidate parent to select the excellent seed production parent on the premise of rich genetic diversity of a sturgeon population, or introduce an exotic parent under the condition of the lower genetic diversity of the sturgeon population. The invention further provides an evaluation method of sturgeon individual genealogy. A genetic relationship between a pair of sturgeon parents and a young sturgeon can be determined according to an identification result. The invention further provides a genetic evaluation method of the sturgeon population. The genetic relationship between the sturgeon parents in the sturgeon population and the young sturgeon can be determined according to an evaluation result. A combination of the 96SNP molecular markers can reflect genetic combination information of different chromosomes and genetic linkage information of the same chromosome, and sufficiently meet genetic detection requirementsin a seed production process.

Description

technical field [0001] The invention relates to the technical field of aquatic organisms, in particular to a group of sturgeon SNP molecular markers, a sturgeon seed production evaluation method and a genetic evaluation method. Background technique [0002] Sturgeon is a general term for fishes of the order Acipenseriformes, belonging to the class Osteichthyes, the subclass Actinopterygii, and the general order Chondrostei. Sturgeon artificial breeding began in the 1980s, but the scale of the industry was once small. Since the beginning of the 21st century, my country's sturgeon aquaculture industry has entered a period of rapid development. The annual output has increased from 9,400 tons in 2003 to 89,800 tons in 2016. The breeding areas cover Beijing, Heilongjiang, Guangdong, Shandong, Yunnan and other 20 In many provinces, the product form has also developed from a single model dominated by caviar in the past to a diversified direction of caviar, fish meat, fish skin and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 赵紫霞江炎亮许建张研
Owner CHINESE ACAD OF FISHERY SCI
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