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A high-efficiency Bacillus amyloliquefaciens and its agent and application

A technology for dissolving Bacillus amyloliquefaciens, applied in application, fungicides, bacteria, etc., can solve the problem of single function of Bacillus amyloliquefaciens, and achieve the effect of improving soil respiration rate and reducing the number of

Active Publication Date: 2019-03-22
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The invention provides a high-efficiency Bacillus amyloliquefaciens, which solves the problem of single function of Bacillus amyloliquefaciens in the prior art

Method used

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  • A high-efficiency Bacillus amyloliquefaciens and its agent and application
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  • A high-efficiency Bacillus amyloliquefaciens and its agent and application

Examples

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Effect test

Embodiment 1

[0048] Example 1. Isolation and identification of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) HW05

[0049] 1. Isolation of Bacillus amyloliquefaciens HW05

[0050] Collect soil samples from the mangrove silt soil in Wenchang, Hainan Province, China, air-dry and mix the soil samples, weigh 20g after sieving and put them into a triangular flask filled with 200mL sterile water and glass beads, fully shake for 10min, and let stand After 5min, dilute to 1×10 by gradient concentration method -2 , 1×10 -3 , each gradient dilution was heated in a water bath at 80°C for 15-20min.

[0051] Prepare a culture medium containing Fusarium oxysporum (Fusarium oxysporum): get Fusarium oxysporum spore suspension (about 1.6×10 5 per mL), draw 500 μL of the spore suspension and apply it to the PDA solid medium in a petri dish with a diameter of 15 cm, and pre-culture for 8-12 h.

[0052] Prepare the medium containing the biocontrol bacterium Paecilomyces lilacinus E16: get the sp...

Embodiment 2

[0064] Embodiment 2, Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) HW05 CGMCCNo.10273 has the activity of resisting streptomycin sulfate and ampicillin

[0065] Through antibiotic labeling experiments, HW05 can be induced to have resistance to streptomycin sulfate and ampicillin at the same time, and can grow stably and maintain antagonism against Fusarium oxysporum on LB medium containing both streptomycin sulfate 300 μg / mL and ampicillin 100 μg / mL activity of pathogens such as bacteria. Because of its resistance to streptomycin sulfate and ampicillin, it can be used together with antibiotics to fight against bacterial contamination in nature, which is conducive to the large-scale fermentation and production of HW05 strains and HW05 bacterial agents, making them useful as bacteria It can resist bacterial pollution in the application of chemical reagents, and it is also beneficial to test the survival ability of HW05 in the field. This is also the characteristic tha...

Embodiment 3

[0066] Embodiment three: the method for preparing bacterial agent by Bacillus amyloliquefaciens HW05 CGMCC No.10273:

[0067] Bacillus amyloliquefaciens HW05CGMCC No.10273 The method for preparing bacterial agents: take the Bacillus amyloliquefaciens HW05 preserved in the LB medium on the slope, streak it onto the LB medium plate, place it at 37°C, and cultivate it for 8 hours. After a single colony grows, Use an inoculation loop to pick HW05 and inoculate it into a Erlenmeyer flask (50mL) containing 20mL of LB culture solution, place it on a shaker, and culture it at 37°C with constant temperature shaking at 160rpm for 2-3h; The inoculum with a percentage content of 1% was placed in a Erlenmeyer flask (500mL) equipped with 500mL LB culture solution; the inoculated shake flask was shaken at a speed of 160rpm at 37°C for 14h to obtain Bacillus amyloliquefaciens HW05 Fermentation liquid, that is, bacterial agent. The bacterial agent of this embodiment contains Bacillus amyloliq...

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Abstract

The invention discloses a strain of high-efficiency Bacillus amyloliquefaciens and its bacterial agent and application, wherein the Latin name of the Bacillus amyloliquefaciens is Bacillus amyloliquefaciens, the strain number is HW05, and it is registered in the General Microorganism Center of China Microbiological Culture Collection Management Committee The entry number is CGMCC No.10273. The high-efficiency bacillus amyloliquefaciens of the present invention has obvious inhibitory effect on Fusarium oxysporum, Phytophthora capsici, Ralstia solanacearum, Pyremia cinerea, Gloosporum anthracnose and root-knot nematode. Application of HW05 alone can effectively prevent and control the foliar diseases of melon powdery mildew, mango anthracnose and mulberry red rust, and the combination of HW05 and Paecilomyces lilacinus E16 has a good synergistic effect on the control of soil-borne banana wilt and banana root-knot nematode. .

Description

technical field [0001] The invention relates to the field of biological control of plant diseases, in particular to a high-efficiency bacillus amyloliquefaciens and its agent and application. Background technique [0002] Crop wilt, bacterial wilt, root rot caused by Fusarium oxysporum, Phytophthora nicotianae, Ralstonia solanacearum, Meloidogyne spp. and root-knot nematode and other devastating soil-borne diseases, as well as those caused by Magnaportheoryzae, Sphaerotheca fuliginea, Colletotrichum gloeosporioides, and Aecidium mori Melon powdery mildew, mango anthracnose, rice blast, mulberry red rust and other foliar diseases have caused huge losses to my country's agricultural production. The use of chemical drugs can have obvious control effects in the short term, but there are a series of problems such as drug resistance and pesticide residues, which can no longer meet people's requirements. The application of chemical drugs in agricultural production is increasingly re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14A01N63/00A01N63/02A01N63/04A01P1/00A01P3/00A01P5/00C12R1/07C12R1/79
CPCA01N63/00A01N63/10A01N63/30C12N1/14C12N1/20C12N1/205C12R2001/07
Inventor 汪军黄俊生梁昌聪周游刘磊杨腊英郭立佳
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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