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Dual Targeting Drug Carriers

A target and drug technology, applied in the field of novel targeted drug carriers, can solve the problems of tumor cell instability, poor effect, slow survival of cancer patients, etc.

Active Publication Date: 2022-06-07
FARMAR LICENSING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, tumor cells are highly unstable and variable, and will develop resistance to drugs during treatment
Various drugs and diagnostic reagents are slow and ineffective in improving the survival of cancer patients

Method used

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  • Dual Targeting Drug Carriers
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  • Dual Targeting Drug Carriers

Examples

Experimental program
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Effect test

specific Embodiment approach

[0031] The present invention provides a dual target drug carrier, comprising a first target molecule and a second target molecule.

[0032] The target molecule of the present invention is a peptide, an antibody or a protein. The target molecules of the present invention can be chemically modified to increase stability. Chemically modified peptides or peptide analogs include any chemical functional equivalent that increases stability and / or efficacy in vivo (in vivo) or in vitro (in vitro). A peptide analog refers to an amino acid derivative of any peptide. Peptide analogs include, but are not limited to, modification of branched chains at the time of synthesis, incorporation of unsaturated amino acids and / or derivatives thereof, cross-linking, and other methods that increase peptide image constraints. For example, branched chain modifications include modifications of amine groups.

[0033] The target molecule can be bound in vivo and in vitro to lesion tissues, especially t...

Embodiment 1

[0043] 1. Preparation of fusion protein bodies

[0044] The EGF, RGD-EGF, RGD4C-EGF, VEGF, RGD-VEGF and RGD4C-VEGF genes were constructed in the expression vector pET28a(+) with Nco I and Xho I restriction enzymes. The C-terminus (ie, carbon-terminus) of RGD and RGD4C is connected to the N-terminus (ie, nitrogen-terminus) of EGF or VEGF, and the C-terminus of EGF or VEGF has His 6 logo. His 6 The markers are helpful for subsequent purification and analysis, wherein the gene sequences of RGD-EGF, RGD4C-EGF, RGD-VEGF and RGD4C-VEGF contain the linker GG. The constructed pET28a(+)EGF, RGD-EGF, RGD4C-EGF, VEGF, RGD-VEGF and RGD4C-VEGF expression vectors were transfected into E.coli.DH5α to produce and preserve vector DNA. The sequence was confirmed to be correct by DNA sequencing.

[0045] 2. Expression and purification of target fusion protein

[0046] The vector of Example 1 was transferred into E. coli BL21 (DE3) by heat shock, and was screened and confirmed with kanamycin...

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Abstract

A dual-target drug carrier is provided, including first and second target molecules, wherein the target molecules include peptides, antibodies or proteins. The target molecule can be combined with specific receptors, specific proteins or glycoproteins, and can also be conjugated with radiotherapy substances to form conjugates.

Description

technical field [0001] The present invention relates to a novel target drug carrier, and particularly relates to a dual target drug carrier with two target molecules. Background technique [0002] Cancer is the leading cause of death in the United States, and cancer mortality rates continue to increase. Cancer is the inability of cells to divide, grow and differentiate normally. The initial clinical manifestations of cancer are extremely uneven, with more than 70 types of cancers occurring in almost all human organs and tissues, and some of these similar cancer types belong to multiple different molecular diseases. Unfortunately, some cancers may be asymptomatic until later in the disease course, making treatment and prognosis extremely difficult. [0003] Cancer treatment usually includes surgery, chemotherapy and / or radiation therapy. All current therapies have serious side effects and reduced quality of life. Most chemotherapy drugs work on both normal and cancerous t...

Claims

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Application Information

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IPC IPC(8): C07K19/00A61K38/00A61P35/00
CPCA61K38/00C07K19/00
Inventor 张正蓝耿立王信二张顺福李佳哲詹佩嘉
Owner FARMAR LICENSING